In situ evaluation of basil oil essential oil cytotoxicity ( Ocimum basilicum L. )

ABSTRACT

Essential oils derived from plants used as condiments represent a large group of natural antimicrobials. Extracts and essential oils from plants have been shown to be efficient in controlling the growth of a wide variety of microorganisms, including filamentous fungi, yeasts and bacteria. Practical uses of these activities are suggested in humans and animals, as well as in the food industry. This study aimed to evaluate the basal basil essential oil (Ocimum basilicum L.) cytotoxicity in NCTC Clone 929 cell line, mouse connective tissue cells (CCIAL 020), which were seeded in Petri dishes and incubated for 48 hours To to form the cell monolayer. For the this test accomplishment of this test, we counted with the collaboration of the Nucleus of Cell Cultures - NCC of the Adolfo Lutz Institute, of the city of São Paulo-SP. The culture medium used is the solid coating medium, and fragments of the samples fragments are were placed on this coating medium and the plates were again incubated for 24 hours. The Biological Reactivity Degree (GR) index, which is the area not stained by the vital dye, is were observed macro and microscopically. Samples were tested in quadruplicates on separate plates. ThusThereunto, the test result showed that the essential oil was cytotoxic in the cell line studied, and that the future work in pharmacology and toxicology needs to be performed to better standardize the therapeutic dose, which is not cytotoxic as the pure oil behaved.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.