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Phytochemical and biological attributes of Bauhinia variegata L. (Caesalpiniaceae) / Atributos fitoquímicos e biológicos de Bauhinia variegata L. (Caesalpiniaceae)
Kamal, Y; Khan, T; Haq, I; Zahra, S. S; Asim, M. H; Shahzadi, I; Mannan, A; Fatima, N.
  • Kamal, Y; COMSATS University Islamabad. Department of Pharmacy. Abbottabad. PK
  • Khan, T; COMSATS University Islamabad. Department of Pharmacy. Abbottabad. PK
  • Haq, I; Quaid-i-Azam University. Department of Pharmacy. PK
  • Zahra, S. S; Shifa Tameer-e-Millat University. Shifa College of Pharmaceutical Sciences. PK
  • Asim, M. H; University of Sargodha. College of Pharmacy. Sargodha. PK
  • Shahzadi, I; COMSATS University Islamabad. Department of Biotechnology. Abbottabad. PK
  • Mannan, A; COMSATS University Islamabad. Department of Pharmacy. Abbottabad. PK
  • Fatima, N; COMSATS University Islamabad. Department of Pharmacy. Abbottabad. PK
Braz. j. biol ; 82: e257990, 2022. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1360195
ABSTRACT
Bauhinia variegata plant is a very popular and traditionally potent ethnomedicine. Therefore, it is need of hour to study ameliorative characteristics of B. variegata for novel secondary metabolites. The current study was designed to explore antiproliferative potential of B. variegata due to scant reports on this aspect. Extracts of various parts (flowers, leaves, bark, stem, and roots) were prepared by successive maceration using organic solvents in increasing order of polarity (n-hexane, ethyl acetate, methanol, and water). The determination of polyphenolic contents was done by using colorimetric methods while antioxidant potential was measured using reducing power assay. Brine shrimp lethality assay was performed for determining preliminary cytotoxicity and antiproliferative activity against breast cancer MCF-7 cell line using MTT protocols. Moreover, antimicrobial activities were detected by using disc diffusion assay. The alpha-amylase assay was performed to monitor the antidiabetic potential of the plant. In case of phytochemical analysis methanolic extract of leaves and bark showed highest phenolic and flavonoids contents. n-Hexane and ethyl acetate extracts of stem and roots exhibited more than 90% mortality with LD50 ranges between 1-25 µg/mL when studied by brine shrimp lethality assay. n-Hexane and ethyl acetate extracts of roots and stem also showed antiproliferative activity against human breast cancer MCF-7 cell line with IC50 values ranges between 12.10-14.20 µg/mL. Most of the extracts displayed moderately high antibacterial and antifungal activities. The n-hexane extract of roots showed antidiabetic activity with 60.80 ± 0.20% inhibition of alpha-amylase. In sum, these preliminary results will be useful for further compound isolation from selected plant parts for the discovery of antibacterial, antidiabetic, and anticancer lead candidates.
RESUMO
A planta Bauhinia variegata é uma etnomedicina muito popular e tradicionalmente potente. Portanto, as características de melhoria de B. variegata foram estudadas. Foi avaliada a determinação dos teores antioxidantes e polifenólicos. O ensaio de letalidade do camarão de salmoura foi realizado para determinar a citotoxicidade preliminar e a atividade antiproliferativa contra linhas de células de câncer de mama MCF-7 usando protocolos de MTT. Além disso, foram detectadas atividades antimicrobianas. O ensaio da alfa-amilase foi realizado para monitorar o potencial antidiabético da planta. Dentre vinte amostras diferentes, o extrato metanólico (EM) da casca apresentou os maiores teores fenólicos totais. A EM das folhas apresentou excelente conteúdo de flavonoides, atividade antioxidante significativa foi exibida pelo extrato hexânico do caule. O extrato do caule de hexano exibe 77,40% como citotóxico em DL50 10,50 µg/mL quando avaliado através do ensaio de letalidade de artêmia. Extratos de hexano e acetato de etila de raiz e caule mostraram atividade antiproliferativa contra a linhagem celular MCF7 de câncer de mama humano (IC50 12,10-14,20 µg/mL). Para potencial antimicrobiano importante, vários extratos exibiram excelentes atividades antibacteriana e antifúngica, enquanto o extrato de n-hexano da raiz mostrou atividade antidiabética (60,80 ± 0,20% na concentração de 200 µg/mL). Em suma, estes resultados preliminares serão úteis para isolamento adicional de compostos a partir de partes de plantas selecionadas para a descoberta de candidatos a antibacterianos, antidiabéticos e anticâncer.

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Full text: Available Index: LILACS (Americas) Main subject: Breast Neoplasms / Bauhinia / Cell Proliferation / MCF-7 Cells / Fabaceae / Anti-Bacterial Agents / Antioxidants Language: English Journal: Braz. j. biol Year: 2022 Type: Article Institution/Affiliation country: COMSATS University Islamabad/PK / Quaid-i-Azam University/PK / Shifa Tameer-e-Millat University/PK / University of Sargodha/PK

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Full text: Available Index: LILACS (Americas) Main subject: Breast Neoplasms / Bauhinia / Cell Proliferation / MCF-7 Cells / Fabaceae / Anti-Bacterial Agents / Antioxidants Language: English Journal: Braz. j. biol Year: 2022 Type: Article Institution/Affiliation country: COMSATS University Islamabad/PK / Quaid-i-Azam University/PK / Shifa Tameer-e-Millat University/PK / University of Sargodha/PK