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DNA damage-related proteins in smokers and non-smokers with oral cancer
Schuch, Lauren Frenzel; De Arruda, José Alcides Almeida; Viana, Karolina Skarlet Silva; Caldeira, Patrícia Carlos; Abreu, Mauro Henrique Nogueira Guimarães; Bernardes, Vanessa Fátima; Aguiar, Maria Cássia Ferreira de.
Affiliation
  • Schuch, Lauren Frenzel; Universidade Federal de Minas Gerais. School of Dentistry. Department of Oral Surgery, Pathology and Clinical Dentistry. Belo Horizonte. BR
  • De Arruda, José Alcides Almeida; Universidade Federal de Minas Gerais. School of Dentistry. Department of Oral Surgery, Pathology and Clinical Dentistry. Belo Horizonte. BR
  • Viana, Karolina Skarlet Silva; Universidade Federal de Minas Gerais. School of Dentistry. Department of Oral Surgery, Pathology and Clinical Dentistry. Belo Horizonte. BR
  • Caldeira, Patrícia Carlos; Universidade Federal de Minas Gerais. School of Dentistry. Department of Oral Surgery, Pathology and Clinical Dentistry. Belo Horizonte. BR
  • Abreu, Mauro Henrique Nogueira Guimarães; Universidade Federal de Minas Gerais. School of Dentistry. Department of Community and Preventive Dentistry. Belo Horizonte. BR
  • Bernardes, Vanessa Fátima; Universidade Federal de Minas Gerais. Biological Sciences Institute. Department of Pathology. Belo Horizonte. BR
  • Aguiar, Maria Cássia Ferreira de; Universidade Federal de Minas Gerais. School of Dentistry. Department of Oral Surgery, Pathology and Clinical Dentistry. Belo Horizonte. BR
Braz. oral res. (Online) ; 36: e027, 2022. tab, graf
Article in En | LILACS-Express | LILACS, BBO | ID: biblio-1360245
Responsible library: BR1.1
ABSTRACT
Abstract Tobacco smoking involves a high risk of human malignancies, including oral cancer, because it contains multiple carcinogens that cause genetic instability. Thus, a worse prognosis would be expected for cancer patients who are smokers. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). Associations between immunoexpression of proteins and clinicopathological data and histopathological grading were also analyzed. A total of 35 individuals (18 non-smokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. The sample consisted of 22 males and 13 females, with a mean age of 63.9±11.8 years. OSCC of non-smokers were well-differentiated tumors in 50% of the cases, and those of smokers were equally distributed into moderately differentiated and poorly differentiated tumors (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.1%) were H2AX-positive, and 23 (65.7%) were P53-positive, with no difference between smokers and non-smokers (p > 0.05). No association was found between proteins and clinicopathologic data (p > 0.05). Similarities in CHK2, H2AX, and P53 immunohistochemical staining patterns were observed between smokers and non-smokers, and immunoexpression was not associated with clinicopathological parameters. However, the findings indicated consistent expression of these proteins in OSCC.
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Full text: 1 Index: LILACS Type of study: Prognostic_studies Language: En Journal: Braz. oral res. (Online) Journal subject: ODONTOLOGIA Year: 2022 Type: Article / Project document

Full text: 1 Index: LILACS Type of study: Prognostic_studies Language: En Journal: Braz. oral res. (Online) Journal subject: ODONTOLOGIA Year: 2022 Type: Article / Project document