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Toxocara canis 30-35 kDa excretory-secretory antigen is an important marker in mice challenged by inocula containing different parasite load levels
Fonseca, Gabriela Rodrigues e; Corral, Marcelo Andreetta; Paula, Fabiana Martins de; Meisel, Dirce Mary Correia Lima; Gryschek, Ronaldo Cesar Borges; Lescano, Susana Angélica Zevallos.
  • Fonseca, Gabriela Rodrigues e; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Corral, Marcelo Andreetta; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Paula, Fabiana Martins de; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Meisel, Dirce Mary Correia Lima; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Gryschek, Ronaldo Cesar Borges; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Lescano, Susana Angélica Zevallos; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
Article in English | LILACS-Express | LILACS | ID: biblio-1360788
ABSTRACT
ABSTRACT The Western-blotting technique was applied to identify antigenic fractions of excretory-secretory Toxocara canis antigen recognized by IgG antibodies throughout an experimental infection in mice challenged by different inocula. Mice were inoculated with 5, 50 and 500 embryonated eggs and serum samples were collected 15, 30, 60, 90 and 120 days post-infection. Serum samples were analyzed using an excretory-secretory Toxocara antigen. Antibodies recognized antigenic fractions from 30 to 90 kDa. The protein fraction of 30-35 kDa was the most frequently recognized regardless of the size of inoculum and the stage of infection represented by the different collection times, but the antigenic recognition was more evident in groups infected with 50 and 500 eggs. This study presents an antigenic panel of the excretory-secretory antigen of T. canis and suggests that the 30-35 kDa antigenic fraction is a promising marker of the infection and should be further explored in future studies on experimental toxocariasis.


Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. Inst. Med. Trop. São Paulo (Online) Journal subject: Medicina Tropical Year: 2022 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. Inst. Med. Trop. São Paulo (Online) Journal subject: Medicina Tropical Year: 2022 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR