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Bioprocess optimization of interferon ß-1-a in Pichia pastoris and its improved inhibitory effect against hepatocellular carcinoma cells
Moatamedi, Nasim; Emamzadeh, Rahman; Sadeghi, Hamid Mir Mohammad; Akbari, Vajihe.
  • Moatamedi, Nasim; Isfahan University of Medical Sciences. Faculty of Pharmacy. Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center. Isfahan. IR
  • Emamzadeh, Rahman; University of Isfahan. Faculty of Sciences. Department of Biology. Isfahan. IR
  • Sadeghi, Hamid Mir Mohammad; Isfahan University of Medical Sciences. Faculty of Pharmacy. Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center. Isfahan. IR
  • Akbari, Vajihe; Isfahan University of Medical Sciences. Faculty of Pharmacy. Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center. Isfahan. IR
Braz. J. Pharm. Sci. (Online) ; 58: e18984, 2022. graf
Article in English | LILACS | ID: biblio-1364429
ABSTRACT
Interferon-ß-1a (INF-ß-1a) has gained significant attention due to its emerging applications in the treatment of different human diseases. Therefore, many researchers have attempted to produce it in large quantities and also in a biologically active form using different expression systems. In the present study, we aimed to improve the expression level of INF-ß-1a by Pichia pastoris using optimization of culture conditions. The codon-optimized INF-ß- 1a gene was cloned into pPICZαA plasmid under the control of alcohol oxidase I (AOX1) promoter. The protein expression was induced using different concentrations of methanol at different pHs and temperatures. The biological activity of produced protein was evaluated by anti-proliferative assay. The ideal culture conditions for the expression of INF-ß-1a by P. pastoris were found to be induction with 2% methanol at pH 7.0 culture medium at 30 C which yielded a concentration of 15.5 mg/L INF-ß-1a in a shake flask. Our results indicate that differences in glycosylation pattern could result in different biological activities as INF- ß-1a produced by P. pastoris could significantly more reduce the cell viability of HepG-2 cells, a hepatocellular carcinoma cell line, than a commercially available form of this protein produced by CHO
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Full text: Available Index: LILACS (Americas) Main subject: Pichia / Interferon-beta / Carcinoma, Hepatocellular / Process Optimization Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2022 Type: Article Affiliation country: Iran Institution/Affiliation country: Isfahan University of Medical Sciences/IR / University of Isfahan/IR

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Full text: Available Index: LILACS (Americas) Main subject: Pichia / Interferon-beta / Carcinoma, Hepatocellular / Process Optimization Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2022 Type: Article Affiliation country: Iran Institution/Affiliation country: Isfahan University of Medical Sciences/IR / University of Isfahan/IR