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Management of Airway Remodeling in a Mouse Model of Allergic Airways Inflammation Using Extracellular Vesicles from Human Bone Marrow-Derived Mesenchymal Stromal Cells
Fragoso, Felipe Yukio Ishikawa; Michelotto, Pedro Vicente; Angulski, Addeli Bez Batti; Leite, Lidiane Maria Boldrini; Senegaglia, Alexandra Cristina; Olandoski, Márcia; Dominguez, Alejandro Correa; Brofman, Paulo Roberto Slud.
  • Fragoso, Felipe Yukio Ishikawa; Pontifícia Universidade Católica do Paraná. School of Medicine. Cell Technology Core. Curitiba. BR
  • Michelotto, Pedro Vicente; Pontifícia Universidade Católica do Paraná. School of Medicine. Cell Technology Core. Curitiba. BR
  • Angulski, Addeli Bez Batti; Instituto Carlos Chagas. Laboratory of Basic Biology of Stem Cells. Fiocruz-Paraná. Curitiba. BR
  • Leite, Lidiane Maria Boldrini; Pontifícia Universidade Católica do Paraná. School of Medicine. Cell Technology Core. Curitiba. BR
  • Senegaglia, Alexandra Cristina; Pontifícia Universidade Católica do Paraná. School of Medicine. Cell Technology Core. Curitiba. BR
  • Olandoski, Márcia; Pontifícia Universidade Católica do Paraná. School of Medicine. Cell Technology Core. Curitiba. BR
  • Dominguez, Alejandro Correa; Instituto Carlos Chagas. Laboratory of Basic Biology of Stem Cells. Fiocruz-Paraná. Curitiba. BR
  • Brofman, Paulo Roberto Slud; Pontifícia Universidade Católica do Paraná. School of Medicine. Cell Technology Core. Curitiba. BR
Braz. arch. biol. technol ; 65: e22200620, 2022. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1364468
ABSTRACT
Abstract Asthma is a chronic respiratory disease affecting 300 million people worldwide. It results in several structural changes in the airways, which are minimally accessible in clinical practice. Cell therapy using mesenchymal stromal cells (MSCs) is a promising strategy for treating asthma due to the paracrine activity of MSCs, which influences tissue regeneration and modulates the immune response. Studies using extracellular vesicles (EV) released by MSCs have demonstrated their regenerative properties in animal models. The aim of this study was to evaluate the potential of EVs isolated from human bone marrow MSCs (hBM-MSCs) to control lung tissue remodeling in ovalbumin-induced allergic asthma in Balb/c mice. We isolated hBM-MSCs from a single donor, expanded and characterized them, and then isolated EVs. Asthma was induced in 43 male Balb/c mice, divided into four groups control, asthmatic (AS), asthmatic plus systemic EVs (EV-S), and asthmatic plus intratracheal EVs (EV-IT). Upon completion of asthma induction, animals were treated with EVs either locally (EV-IT) or intravenously (EV-S). Seven days after, we performed bronchoalveolar lavage (BAL) and the total nuclear cells were counted. The animals were euthanized, and the lungs were collected for histopathological analysis of the airways. The EV-S group showed improvement in only the total BAL cell count compared with the AS group, while the EV-IT group showed significant improvement in almost all evaluated criteria. Therefore, we demonstrate that the local application of EVs derived from hBM-MSCs may be a potential treatment in controlling asthma.


Full text: Available Index: LILACS (Americas) Type of study: Prognostic study Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2022 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Carlos Chagas/BR / Pontifícia Universidade Católica do Paraná/BR

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Full text: Available Index: LILACS (Americas) Type of study: Prognostic study Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2022 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Carlos Chagas/BR / Pontifícia Universidade Católica do Paraná/BR