Circ_0075825 promotes gastric cancer progression via adsorbing miR-432-5p to modulate SOX9
Clinics
;
77: 100018, 2022. tab, graf
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1375179
ABSTRACT
Abstract Methods Circ_0075825 expression in adjacent tissues and GC tissues was evaluated by bioinformatics method and quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). How circ_0075825 regulated GC cell growth, migration, invasion, and apoptosis were investigated by cell counting kit-8 assay, transwell assay and flow cytometry. The targeted interplays among circ_0075825, and miR-432-5p and Sex-Determining Region Y-related high-mobility group box 9 (SOX9) were explored by bioinformatics analysis and luciferase reporter gene assay. The regulatory effects of circ_0075825 and miR-432-5p on SOX9 protein expression were probed by western blot. Results Circ_0075825 expression was raised in GC tissues and cell lines. Circ_0075825 overexpression promoted the proliferative, migrative and invasive abilities of GC cells, while inhibiting apoptosis, while depletion of circ_0075825 suppressed the malignant biological behaviors of GC cells. SOX9 was identified as one of the direct target genes of miR-432-5p, and circ_0075825 repressed the expression of miR-432-5p, to induce the expression of SOX9. Furthermore, miR-432-5p overexpression counteracted the promoting effect of circ_0075825 on the malignancy of GC cells. Conclusion Circ_0075825 promotes GC progression via sponging miR-432-5p to regulate SOX9 expression level, and it may be a novel therapeutic target for treating GC.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2022
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
The First Affiliated Hospital of Hainan Medical University/CN
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