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MicroRNA-128b mediates lipopolysaccharide-induced apoptosis via reactive oxygen species in human pulmonary microvascular endothelial cells
Long, Guangwen; Yang, Xiulin; Ji, Chunling; Dong, Yukang.
  • Long, Guangwen; Guizhou Provincial Peoples Hospital. Emergency Department of Internal Medicine. CN
  • Yang, Xiulin; Guizhou Provincial Peoples Hospital. Emergency Department of Internal Medicine. CN
  • Ji, Chunling; Guizhou Provincial Peoples Hospital. Emergency Department of Internal Medicine. CN
  • Dong, Yukang; Guizhou Provincial Peoples Hospital. Emergency Department of Internal Medicine. CN
Clinics ; 77: 100020, 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1375186
ABSTRACT
ABSTRACT

Objectives:

This study aimed to explore the effects of miR-128b in the regulation of Lipopolysaccharide (LPS) induced apoptosis.

Methods:

Human Pulmonary Microvascular Endothelial Cells (HPMECs) were transfected with an miR-128b inhibitor and stimulated with LPS for 24 h. FCM was performed to detect apoptosis and Reactive Oxygen Species (ROS) production. In addition, miRNA and caspase-3 expression levels were determined using real-time quantitative polymerase chain reaction and western blotting.

Results:

LPS significantly induced apoptosis and ROS production and upregulated miR-128b and caspase-3 expressions in HPMECs. However, LPS-induced effects were suppressed when an miR-128b inhibitor was used. Preincu-bation with NAC decreased the LPS-induced apoptosis of HPMECs.

Conclusions:

These effects were mediated by miR-128b via the caspase-3 pathway.


Full text: Available Index: LILACS (Americas) Language: English Journal: Clinics Journal subject: Medicine Year: 2022 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Guizhou Provincial Peoples Hospital/CN

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Full text: Available Index: LILACS (Americas) Language: English Journal: Clinics Journal subject: Medicine Year: 2022 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Guizhou Provincial Peoples Hospital/CN