MicroRNA-128b mediates lipopolysaccharide-induced apoptosis via reactive oxygen species in human pulmonary microvascular endothelial cells
Clinics
;
77: 100020, 2022. tab, graf
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1375186
ABSTRACT
ABSTRACT Objectives:
This study aimed to explore the effects of miR-128b in the regulation of Lipopolysaccharide (LPS) induced apoptosis.Methods:
Human Pulmonary Microvascular Endothelial Cells (HPMECs) were transfected with an miR-128b inhibitor and stimulated with LPS for 24 h. FCM was performed to detect apoptosis and Reactive Oxygen Species (ROS) production. In addition, miRNA and caspase-3 expression levels were determined using real-time quantitative polymerase chain reaction and western blotting.Results:
LPS significantly induced apoptosis and ROS production and upregulated miR-128b and caspase-3 expressions in HPMECs. However, LPS-induced effects were suppressed when an miR-128b inhibitor was used. Preincu-bation with NAC decreased the LPS-induced apoptosis of HPMECs.Conclusions:
These effects were mediated by miR-128b via the caspase-3 pathway.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2022
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Guizhou Provincial Peoples Hospital/CN
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