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Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)
Costa, Maria Beatriz Silva; Simões, Rejane de Castro; Silva, Márcia de Jesus Amazonas da; Oliveira, André Correa de; Acho, Leonard Domingo Rosales; Lima, Emerson Silva; Tadei, Wanderli Pedro; Teles, Helder Lopes; Oliveira, Camila Martins de.
  • Costa, Maria Beatriz Silva; Universidade Federal do Amazonas. Instituto de Ciências Exatas e Tecnologias. Itacoatiara. BR
  • Simões, Rejane de Castro; Instituto Nacional de Pesquisas do Amazonas. Laboratório de Malária e Dengue. Manaus. BR
  • Silva, Márcia de Jesus Amazonas da; Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus. BR
  • Oliveira, André Correa de; Instituto Nacional de Pesquisas do Amazonas. Laboratório de Malária e Dengue. Manaus. BR
  • Acho, Leonard Domingo Rosales; Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus. BR
  • Lima, Emerson Silva; Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus. BR
  • Tadei, Wanderli Pedro; Instituto Nacional de Pesquisas do Amazonas. Laboratório de Malária e Dengue. Manaus. BR
  • Teles, Helder Lopes; Universidade Federal de Rondonópolis. Instituto de Ciências Exatas e Naturais. Rondonópolis. BR
  • Oliveira, Camila Martins de; Universidade Federal de Rondonópolis. Instituto de Ciências Exatas e Naturais. Rondonópolis. BR
Rev. Soc. Bras. Med. Trop ; 55: e0373, 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1376351
ABSTRACT
ABSTRACT

Background:

Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the risk of resistance in these mosquitoes. One possible mechanism is uncontrolled production of reactive oxygen species (ROS) in the larvae, which can cause changes at the cellular level. Thus, the crude extract of Xylaria sp. was evaluated to investigate the oxidative effect of this extract in A. aegypti larvae by quantifying the oxidative damage to proteins and lipids.

Methods:

The larvicidal potential of the crude extract of Xylaria sp. Was evaluated, and the extract was subsequently tested in human lung fibroblasts for cytotoxicity and ROS production. ROS level was quantified in the larvae that were killed following exposure to the extract in the larvicide test.

Results:

The crude extract of Xylaria sp. Caused cytotoxicity and induced ROS production in human lung fibroblasts and A. aegypti larvae, respectively. In the larvicide trial, the extract showed an LC50 of 264.456 ppm and an LC90 of 364.307 ppm, and was thus considered active. The extract showed greater oxidative damage to lipids and proteins, with LC90 values of 24.7 µmol MDA/L and 14.6278 ×10-3 nmol carbonyl/ mg protein, respectively.

Conclusions:

Crude extracts of Xylaria sp. induced oxidative stress that may have caused the mortality of A. aegypti larvae.


Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2022 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Nacional de Pesquisas do Amazonas/BR / Universidade Federal de Rondonópolis/BR / Universidade Federal do Amazonas/BR

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Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2022 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Nacional de Pesquisas do Amazonas/BR / Universidade Federal de Rondonópolis/BR / Universidade Federal do Amazonas/BR