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Purified novel and new diferuloyl glycerate related phenolic acid from Pandanus odoratissimus flowers shows antioxidant, invertase inhibition and control against diabetic foot ulcer (DFU) causing bacterial pathogens - An in vitro study to establish an effective regulation over type 2 diabetes mellitus
Suvetha, Sivamani Palanisamy; Sathishkumar, Thiyagarajan; Kumaraesan, Kuppamuthu; Rapheal, Vinohar Stephen; Muthukumaran, Velayutham; Thirugnanam, Natarajan.
  • Suvetha, Sivamani Palanisamy; Kumaraguru College of Technology. Department of Biotechnology. Coimbatore. IN
  • Sathishkumar, Thiyagarajan; Kumaraguru College of Technology. Department of Biotechnology. Coimbatore. IN
  • Kumaraesan, Kuppamuthu; Kumaraguru College of Technology. Department of Biotechnology. Coimbatore. IN
  • Rapheal, Vinohar Stephen; Kumaraguru College of Technology. Department of Biotechnology. Coimbatore. IN
  • Muthukumaran, Velayutham; Kumaraguru College of Technology. Department of Mechanical Engineering. Coimbatore. IN
  • Thirugnanam, Natarajan; Sri Ramakrishna Hospital. Chief Plastic Surgeon. Coimbatore. IN
Braz. J. Pharm. Sci. (Online) ; 58: e19484, 2022. tab, graf
Article in English | LILACS | ID: biblio-1383994
ABSTRACT
Abstract Chronic type 2 diabetes mellitus (T2DM) and its associated diseases are major concern among human population and also responsible for significant mortality rate. Hence, the present study aims to evaluate and correlate the invertase inhibition, antioxidant activity and control against DFU causing bacterial pathogens by Pandanus odoratissimus flowers. Two dimensional preparative thin layer chromatography (2D PTLC) was adopted to purify the phenolic acid component and LC-MS2 was done to predict the phenolic acid structures. Standard spectrophotometry methods were adopted to investigate the in vitro invertase inhibitory and antioxidant (CUPRAC and ABTS) activities. Agar well diffusion and broth dilution assays were used to record the antibacterial property against DFU causing pathogens isolated from clinical samples. Statistical analyses were used to validate the experiments. A new and novel diferuloyl glycerate related phenolic acid (m/z 442) purified from PTLC eluate has recorded satisfactory cupric ion reducing power (ED50= 441.4±2.5 µg), moderate ABTS radical scavenging activity (IC50= 450.3±10 µg; 32.5±1.5%), and a near moderate, in vitro, invertase mixed type inhibition (24.5±4.5%; Ki 400 µg). Similarly, bacterial growth inhibitory kinetics has showed a significant inhibition against E. coli and S. aureus.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: In Vitro Techniques / Diabetic Foot / Pandanaceae / Flowers / Beta-Fructofuranosidase / Diabetes Mellitus, Type 2 Limits: Female / Humans / Male Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2022 Type: Article Affiliation country: India Institution/Affiliation country: Kumaraguru College of Technology/IN / Sri Ramakrishna Hospital/IN

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Full text: Available Index: LILACS (Americas) Main subject: In Vitro Techniques / Diabetic Foot / Pandanaceae / Flowers / Beta-Fructofuranosidase / Diabetes Mellitus, Type 2 Limits: Female / Humans / Male Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2022 Type: Article Affiliation country: India Institution/Affiliation country: Kumaraguru College of Technology/IN / Sri Ramakrishna Hospital/IN