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Evaluation of miR-15a, miR-16-1, ZAP-70, Ang-2, and Bcl-2 as potential prognostic biomarkers in chronic lymphocytic leukemia
Braga, Tatiane Vieira; Evangelista, Fernanda Cristina Gontijo; Santiago, Marie Gabriele; Ferrão, Aline Lúcia Menezes; Almeida, Tamara Dauare de; Barbosa, Bárbara Lima da Fonseca; Araujo, Sergio Schusterschitz da Silva; Ribeiro, Glaciano Nogueira; Carvalho, Maria das Graças; Sabino, Adriano de Paula.
  • Braga, Tatiane Vieira; Federal University of Minas Gerais. School of Pharmacy. Clinical and Toxicological Analysis Department. Belo Horizonte. BR
  • Evangelista, Fernanda Cristina Gontijo; Federal University of Minas Gerais. School of Pharmacy. Clinical and Toxicological Analysis Department. Belo Horizonte. BR
  • Santiago, Marie Gabriele; Federal University of Minas Gerais. School of Pharmacy. Clinical and Toxicological Analysis Department. Belo Horizonte. BR
  • Ferrão, Aline Lúcia Menezes; Federal University of Minas Gerais. School of Pharmacy. Clinical and Toxicological Analysis Department. Belo Horizonte. BR
  • Almeida, Tamara Dauare de; Federal University of Minas Gerais. School of Pharmacy. Clinical and Toxicological Analysis Department. Belo Horizonte. BR
  • Barbosa, Bárbara Lima da Fonseca; Federal University of Minas Gerais. School of Pharmacy. Clinical and Toxicological Analysis Department. Belo Horizonte. BR
  • Araujo, Sergio Schusterschitz da Silva; Federal University of Minas Gerais. Clinical Hospital. Belo Horizonte. BR
  • Ribeiro, Glaciano Nogueira; Federal University of Minas Gerais. Clinical Hospital. Belo Horizonte. BR
  • Carvalho, Maria das Graças; Federal University of Minas Gerais. School of Pharmacy. Clinical and Toxicological Analysis Department. Belo Horizonte. BR
  • Sabino, Adriano de Paula; Federal University of Minas Gerais. School of Pharmacy. Clinical and Toxicological Analysis Department. Belo Horizonte. BR
Braz. J. Pharm. Sci. (Online) ; 58: e19332, 2022. tab, graf
Article in English | LILACS | ID: biblio-1384002
ABSTRACT
Chronic lymphocytic leukemia (CLL) is a blood cancer characterized by the accumulation of clonal B-lymphocytes. This study evaluated the mRNA gene expression of miR-15a, miR-16- 1, ZAP-70, and Ang-2 by qPCR, as well as the plasma levels of Bcl-2 by Elisa immunoassay, in CLL patients and healthy controls. Significant differences were observed when comparing patients and controls regarding miR-15a (p < 0.001), miR-16-1 (p < 0.001) mRNA, Ang-2 gene expression, and Bcl-2 plasma levels (p < 0.001). When stratified by risk, differences were maintained with a significantly reduced expression in high-risk patients. A positive correlation was observed between miR-15a and platelets (R2 = 0.340; p = 0.009) as well as between Bcl-2 and leukocytes (R2 = 0.310; p = 0.019). Conversely, negative correlations were observed between ZAP-70 and platelets (R2 = - 0.334; p = 0.011), between miR-15a and lymphocytes (R2 = - 0.376; p = 0.004), as well as between miR-16-and lymphocytes (R2 = - 0.515; p = 0.00004). The data suggest that a reduction in miR-15a and miR-16-1 expressions, in addition to an overexpression of Bcl-2, are associated with the reduction in apoptosis and, consequently, to a longer survival of lymphocytes, thus contributing to lymphocyte accumulation and aggravation of the disease. By contrast, Ang-2 expression was significantly higher in A than in B + C Binet groups. This context leads to the speculation that this biomarker should be investigated in more robust studies within populations with a still relevantly indolent form of the disease in an attempt to identify those patients with a greater potential for an aggravation of the disease
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Full text: Available Index: LILACS (Americas) Main subject: Biomarkers / Leukemia, Lymphocytic, Chronic, B-Cell / ZAP-70 Protein-Tyrosine Kinase Type of study: Prognostic study Limits: Female / Humans / Male Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2022 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Minas Gerais/BR

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Full text: Available Index: LILACS (Americas) Main subject: Biomarkers / Leukemia, Lymphocytic, Chronic, B-Cell / ZAP-70 Protein-Tyrosine Kinase Type of study: Prognostic study Limits: Female / Humans / Male Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2022 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Minas Gerais/BR