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Genotyping of Listeria monocytogenes isolates by high-resolution melting curve (HRM) analysis of tandem repeat locus
Narimisa, Negar; Amraei, Fatemeh; Sholeh, Mohammad; Mirkalantari, Shiva; Razavi, Shabnam; Kalani, Behrooz Sadeghi; Lotfollahi, Lida; Jazi, Faramarz Masjedian.
  • Narimisa, Negar; Iran University of Medical Science. Microbial Biotechnology Research Center. Tehran. IR
  • Amraei, Fatemeh; Iran University of Medical Science. Microbial Biotechnology Research Center. Tehran. IR
  • Sholeh, Mohammad; Iran University of Medical Science. Microbial Biotechnology Research Center. Tehran. IR
  • Mirkalantari, Shiva; Iran University of Medical Science. Microbial Biotechnology Research Center. Tehran. IR
  • Razavi, Shabnam; Iran University of Medical Science. Microbial Biotechnology Research Center. Tehran. IR
  • Kalani, Behrooz Sadeghi; Ilam University of Medical Sciences. Department of Medical Microbiology. Faculty of Medicine. Ilam. IR
  • Lotfollahi, Lida; Urmia University of Medical Sciences. Department of Microbiology. Faculty of Medicine. Urmia. IR
  • Jazi, Faramarz Masjedian; Iran University of Medical Science. Microbial Biotechnology Research Center. Tehran. IR
Braz. j. infect. dis ; 26(2): 102348, 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1384117
ABSTRACT
Abstract Listeria monocytogenes is responsible for causing listeriosis, a type of food poisoning with high mortality. This bacterium is mainly transmitted to humans through the consumption of contaminated foods. Detection of L. monocytogenes through molecular methods is crucial for food safety and clinical diagnosis. Present techniques are characterized by low discrimination power and high cost, as well as being time-consuming and taking several days to give the final result. In our study, MLVA-HRM (Multiple-Locus Variable-number tandem repeats Analysis ‒ High-Resolution Melting) was investigated as an alternative method for a fast and precise method for the genotyping of L. monocytogenes isolates. Forty-eight isolates of L. monocytogenes obtained from the microbial bank of Department of Microbiology, Iran University of Medical Sciences, were typed by MLVA-HRM analysis using five Variable Numbers of Tandem Repeat (VNTR) loci. A total of 43 different types were obtained. This research demonstrated the usefulness of the MLVA-HRMA method and its ability to discriminate L. monocytogenes isolates. Since this method is easier and more efficient than existing methods, it can be widely used in food processing plants and diagnostic laboratories as a fast and accurate method.


Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2022 Type: Article Affiliation country: Iran Institution/Affiliation country: Ilam University of Medical Sciences/IR / Iran University of Medical Science/IR / Urmia University of Medical Sciences/IR

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Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2022 Type: Article Affiliation country: Iran Institution/Affiliation country: Ilam University of Medical Sciences/IR / Iran University of Medical Science/IR / Urmia University of Medical Sciences/IR