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Marein regulates insulin resistance in diabetic nephropathy mice by inducing autophagy / Marein regula la resistencia a la insulina en ratones con nefropatía diabética induciendo la autofagia
Li, Tian; Abula, Zulipiya; kadier, Kedireya; Guo, Yanli; Wang, Lifeng; Zhang, Yongwei; Ran, Zheng; Song, Zhipeng; Zhang, Fang; Mao, Xinmin.
  • Li, Tian; Xinjiang Medical University. School of Basic Medical Sciences. Department of Histology and Embryology. Urumqi. CN
  • Abula, Zulipiya; Xinjiang Medical University. College of Traditional Chinese Medicine. Urumqi. CN
  • kadier, Kedireya; Xinjiang Medical University,. Pharmacy College. Department of Pharmacology. Urumqi. CN
  • Guo, Yanli; Xinjiang Medical University,. Pharmacy College. Department of Pharmacology. Urumqi. CN
  • Wang, Lifeng; Xinjiang Medical University. School of Basic Medical Sciences. Department of Physiology. Urumqi. CN
  • Zhang, Yongwei; Xinjiang Medical University. College of Traditional Chinese Medicine. Urumqi. CN
  • Ran, Zheng; Xinjiang Medical University. Pharmacy College. Department of Pharmacology. Urumqi. CN
  • Song, Zhipeng; Xinjiang Medical University. Pharmacy College. Department of Pharmacology. Urumqi. CN
  • Zhang, Fang; Xinjiang Medical University. School of Basic Medical Sciences. Department of Histology and Embryology. Urumqi. CN
  • Mao, Xinmin; Xinjiang Medical University. College of Traditional Chinese Medicine. Urumqi. CN
Int. j. morphol ; 39(6): 1635-1645, dic. 2021.
Article in English | LILACS | ID: biblio-1385530
ABSTRACT

SUMMARY:

Marein is the main active substance of Coreopsis tinctoria nutt. It not only has anti-oxidation and anti-tumor effects, but also can lower blood lipid, prevent high blood glucose, improve insulin resistance, inhibit gluconeogenesis and promote glycogen synthesis. However, the exact mechanism of its action is still unclear. Here, we explored the effect and mechanism of Marein on insulin resistance. The mice were divided into db/m, db/db, metformin+db/db, and marein+db/db groups. The body weight and kidney weight were recorded. Serum biochemical and renal function tests were measured after 8 weeks of continuous administration. Kidney tissues were subjected to HE staining, PAS staining, and Masson staining. The effect of marein on PI3K/Akt signal and autophagy pathway was detected by Western blot. After 8 weeks of Marein intervention, the body weight and kidney weight of mice did not change significantly, but the fasting blood glucose and blood lipid levels were significantly reduced than db/db group. Marein significantly improved the insulin resistance index, increased serum adiponectin and improved glucose and lipid metabolism disorders of db/db mice. Moreover, marein improved the basement membrane thickness of glomeruli and tubules, improved glomerular sclerosis and tubular fibrosis, as well as renal insufficiency, thereby protecting kidney function and delaying the pathological damage. Furthermore, marein increased the expression of PI3K and the phosphorylation of Akt/Akt (Ser473), and promoted the expression of LC3II/I, Beclin1 and ATG5. Additionally, it promoted the expression of FGFR1 in the kidney of db/db mice, and promoted the increase of serum FGF21 and FGF23. Marein has a protective effect on the kidneys of diabetic mice. It protects diabetic nephropathy by regulating the IRS1/PI3K/Akt signaling pathway to improve insulin resistance. Therefore, marein may be an insulin sensitizer.
RESUMEN
RESUMEN Marein es la principal sustancia activa de Coreopsis tinctoria nutt. No solo tiene efectos antioxidantes y antitumorales, sino que también puede reducir los lípidos en sangre, prevenir la glucemia alta, mejorar la resistencia a la insulina, inhibir la gluconeogénesis y promover la síntesis de glucógeno. Sin embargo, el mecanismo exacto de su acción aún no está claro. Se analizó el efecto y el mecanismo de Marein sobre la resistencia a la insulina. Los ratones se dividieron en grupos db / m, db / db, metformina + db / db y mareína + db / db. Se registró el peso corporal y el peso de los riñones. Se midieron las pruebas de función renal y bioquímica sérica después de 8 semanas de administración continua. Los tejidos renales se sometieron a tinción HE, tinción PAS y tinción Masson. El efecto de la mareína sobre la señal de PI3K / Akt y la vía de autofagia se detectó mediante Western blot. Al término de 8 semanas de tratamiento con mareína, el peso corporal y el peso de los riñones de los ratones no cambiaron significativamente, pero los niveles de glucosa en sangre y lípidos en sangre en ayunas se redujeron significativamente en relación a los del grupo db / db. Marein mejoró significativamente el índice de resistencia a la insulina, aumentó la adiponectina sérica y mejoró los trastornos del metabolismo de la glucosa y los lípidos de los ratones db / db. Además, la mareína mejoró el grosor de la membrana basal de los glomérulos y túbulos, mejoró la esclerosis glomerular y la fibrosis tubular, así como la insuficiencia renal, protegiendo la función renal y retrasando el daño patológico. Además, la mareína aumentó la expresión de PI3K y la fosforilación de Akt / Akt (Ser473), y promovió la expresión de LC3II / I, Beclin1 y ATG5. Además, promovió la expresión de FGFR1 en el riñón de ratones db / db y el aumento de FGF21 y FGF23 en suero. Marein tiene un efecto protector sobre los riñones de ratones diabéticos. Protege la nefropatía diabética regulando la vía de señalización IRS1 / PI3K / Akt para mejorar la resistencia a la insulina. Por tanto, la mareína puede ser un sensibilizador a la insulina.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Insulin Resistance / Chalcones / Diabetic Nephropathies Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Xinjiang Medical University/CN / Xinjiang Medical University,/CN

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Full text: Available Index: LILACS (Americas) Main subject: Insulin Resistance / Chalcones / Diabetic Nephropathies Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Xinjiang Medical University/CN / Xinjiang Medical University,/CN