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Effect of kefir on increased apoptosis in liver and kidney in cisplatin toxicity / Efecto del kéfir sobre el aumento de la apoptosis en el hígado y los riñones en la toxicidad del cisplatino
Sah, Hüseyin; Gülmez, Nurhayat; Söyler, Gizem; Sayiner, Serkan; Sehirli, Ahmet Özer; Kükner, Aysel.
  • Sah, Hüseyin; Near East University. Faculty of Veterinary Medicine. Department of Histology and Embryology. Nicosia. CY
  • Gülmez, Nurhayat; Siirt University. Faculty of Veterinary Medicine. Department of Histology and Embryology. TR
  • Söyler, Gizem; Koç University. Graduate School of Health Sciences and School of Medicine. Istanbul. TR
  • Sayiner, Serkan; Near East University. Faculty of Veterinary Medicine. Department of Biochemistry. Nicosia. CY
  • Sehirli, Ahmet Özer; Near East University. Faculty of Dentistry. Department of Pharmacology. Nicosia. CY
  • Kükner, Aysel; Near East University. Faculty of Medicine. Department of Histology and Embryology. Nicosia. CY
Int. j. morphol ; 40(2): 480-488, 2022. ilus, tab
Article in English | LILACS | ID: biblio-1385631
ABSTRACT

SUMMARY:

Cisplatin is a chemotherapeutic agent inducing liver and kidney damage. In this study, we intended to investigate the impact of kefir beverage, an essential probiotic and functional food, on liver and kidney damage induced by cisplatin. Wistar albino rats were divided into four groups Control, Cisplatin (single dose of 7 mg/kg, intraperitoneal), Kefir (2 ml/d, 7 d, oral gavage), and Cisplatin+Kefir (CK). At the end of day 7, animals were euthanized. Blood, kidney, and liver tissue samples were collected. For both tissues, biochemically ALT, AST, Urea, Creatine; histomorphologically, hematoxylin-eosin, Masson's Trichrome, and immunohistochemical staining of caspase-3, a marker of apoptosis, were performed. Serum urea and creatinine levels of the Cisplatin group were significantly higher than the Control group (p<0.05). In the CK group, kefir consumption decreased urea and creatinin levels approached to Control and Kefir groups. Cisplatin resulted in higher ALT and AST activities, indicating hepatocellular damage, compared to the Control group (p<0.05). Kefir consumption decreased ALT activities approached to both the Control and Kefir group. Histomorphological observations were in agreement biochemical results. In liver and kidney tissues, structural damage was observed with an increase in collagen fibers in the Cisplatin group, and Caspase-3 activity was immunohistochemically higher than in the other groups. In the CK group, collagen fiber increase, structural damage, and Caspase-3 activities were less than in the Cisplatin group. Kefir consumption alleviated liver and kidney damage. However, more research is required to understand such effect of kefir better.
RESUMEN
RESUMEN El cisplatino es un agente quimioterapéutico que induce daño hepático y renal. En este estudio, intentamos investigar el efecto del kéfir, un alimento funcional y probiótico esencial, en el daño hepático y renal inducido por el cisplatino. Se dividieron ratas albinas Wistar en cuatro grupos control, cisplatino (dosis única de 7 mg/kg, intraperitoneal), kéfir (2 ml/día, 7 días, sonda oral) y cisplatino + kéfir (CK). Al final del día 7, los animales fueron sacrificados. Se recolectaron muestras de sangre, riñón y tejido hepático. Se determinó ALT, AST, Urea y Creatina; Para el análisis histomorfológico, se realizaron tinciones con hematoxilina-eosina, tricrómico de Masson y para inmunohistoquímica, caspasa-3, un marcador de apoptosis. Los niveles séricos de urea y creatinina del grupo de cisplatino fueron significativamente más altos que los del grupo de control (p<0,05). En el grupo CK, el consumo de kéfir disminuyó los niveles de urea y creatinina acercándose a los grupos Control y Kéfir. El cisplatino resultó en actividades más altas de ALT y AST, lo que indica daño hepatocelular, en comparación con el grupo Control (p<0.05). El consumo de kéfir disminuyó las actividades de ALT tanto en el grupo Control como en el de Kéfir. Las observaciones histomorfológicas coincidieron con los resultados bioquímicos. En tejidos hepáticos y renales se observó daño estructural con aumento de fibras colágenas en el grupo de Cisplatino, y la actividad de Caspasa-3 fue inmunohistoquímicamente mayor que en los otros grupos. En el grupo de CK, el aumento de las fibras colágenas, el daño estructural y las actividades de Caspasa-3 fueron menores que en el grupo Cisplatino. El consumo de kéfir mejoró el daño hepático y renal. Sin embargo, se requiere más investigación para comprender mejor el efecto del kéfir.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Cisplatin / Apoptosis / Kefir / Kidney / Liver Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2022 Type: Article Affiliation country: Cyprus / Turkey Institution/Affiliation country: Koç University/TR / Near East University/CY / Siirt University/TR

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Full text: Available Index: LILACS (Americas) Main subject: Cisplatin / Apoptosis / Kefir / Kidney / Liver Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2022 Type: Article Affiliation country: Cyprus / Turkey Institution/Affiliation country: Koç University/TR / Near East University/CY / Siirt University/TR