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Antibacterial and Antiadhesion Effects of Psidium guajava Fractions on a Multispecies Biofilm Associated with Periodontitis
Gómez, Pablo Alejandro Millones; Mendizábal, Margarita Fe Requena; Poma, Roger Damaso Calla; Cifuentes, Tania Valentina Rosales; Quispe, Federico Martin Malpartida; Torres, Dora Jesús Maurtua; Amaranto, Reyma Evelyn Bacilio; Medina, Carlos Alberto Minchón; Contreras, Lusin Antonio Ponce.
  • Gómez, Pablo Alejandro Millones; Universidad Señor de Sipán. Escuela de Medicina Humana. Chiclayo. PE
  • Mendizábal, Margarita Fe Requena; Universidad Nacional Mayor de San Marcos. Faculty of Dentistry. Lima. PE
  • Poma, Roger Damaso Calla; Universidad Nacional Mayor de San Marcos. Faculty of Dentistry. Lima. PE
  • Cifuentes, Tania Valentina Rosales; Universidad Nacional Mayor de San Marcos. Faculty of Dentistry. Lima. PE
  • Quispe, Federico Martin Malpartida; Universidad Privada Norbert Wiener. Faculty of Health Sciences. Lima. PE
  • Torres, Dora Jesús Maurtua; Universidad Peruana Cayetano Heredia. Faculty of Science. Lima. PE
  • Amaranto, Reyma Evelyn Bacilio; Universidad Peruana Cayetano Heredia. Faculty of Science. Lima. PE
  • Medina, Carlos Alberto Minchón; Universidad Nacional de Trujillo. Facultad de Ciencias Físicas y Matemáticas. Trujillo. PE
  • Contreras, Lusin Antonio Ponce; Universidad Nacional Mayor de San Marcos. Faculty of Dentistry. Lima. PE
Pesqui. bras. odontopediatria clín. integr ; 22: e210080, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1386816
ABSTRACT
Abstract

Objective:

To assess the antibacterial activity of Psidium guajava fractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis in vitro. Material and

Methods:

Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajava was evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobial susceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey multiple comparison test.

Results:

The rank-based Kruskal-Wallis test highlighted differences in the effects of the fractions on the zone of inhibition for each oral bacterium, including S. gordonii (p=0.000), F. nucleatum (p=0.000), and P. gingivalis (p=0.000), the Tukey test showed that the group treated with 0.12% chlorhexidine exhibited the least amount of adhesion, followed by the group treated with the 1.56 mg/mL methanol fraction.

Conclusion:

The methanol fraction of P. guajava had an antibacterial effect on S. gordonii and P. gingivalis, and the 1.56 mg/mL methanol fraction decreased biofilm adhesion.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Biofilms / Psidium / Streptococcus gordonii / Anti-Bacterial Agents Type of study: Evaluation studies / Risk factors Language: English Journal: Pesqui. bras. odontopediatria clín. integr Journal subject: Dentistry Year: 2022 Type: Article Affiliation country: Peru Institution/Affiliation country: Universidad Nacional Mayor de San Marcos/PE / Universidad Nacional de Trujillo/PE / Universidad Peruana Cayetano Heredia/PE / Universidad Privada Norbert Wiener/PE / Universidad Señor de Sipán/PE

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Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Biofilms / Psidium / Streptococcus gordonii / Anti-Bacterial Agents Type of study: Evaluation studies / Risk factors Language: English Journal: Pesqui. bras. odontopediatria clín. integr Journal subject: Dentistry Year: 2022 Type: Article Affiliation country: Peru Institution/Affiliation country: Universidad Nacional Mayor de San Marcos/PE / Universidad Nacional de Trujillo/PE / Universidad Peruana Cayetano Heredia/PE / Universidad Privada Norbert Wiener/PE / Universidad Señor de Sipán/PE