Antibacterial and Antiadhesion Effects of Psidium guajava Fractions on a Multispecies Biofilm Associated with Periodontitis
Pesqui. bras. odontopediatria clín. integr
;
22: e210080, 2022. tab, graf
Article
in English
| LILACS, BBO
| ID: biblio-1386816
ABSTRACT
Abstract Objective:
To assess the antibacterial activity of Psidium guajava fractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis in vitro. Material andMethods:
Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajava was evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobial susceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey multiple comparison test.Results:
The rank-based Kruskal-Wallis test highlighted differences in the effects of the fractions on the zone of inhibition for each oral bacterium, including S. gordonii (p=0.000), F. nucleatum (p=0.000), and P. gingivalis (p=0.000), the Tukey test showed that the group treated with 0.12% chlorhexidine exhibited the least amount of adhesion, followed by the group treated with the 1.56 mg/mL methanol fraction.Conclusion:
The methanol fraction of P. guajava had an antibacterial effect on S. gordonii and P. gingivalis, and the 1.56 mg/mL methanol fraction decreased biofilm adhesion.
Full text:
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Index:
LILACS (Americas)
Main subject:
Periodontitis
/
Biofilms
/
Psidium
/
Streptococcus gordonii
/
Anti-Bacterial Agents
Type of study:
Evaluation studies
/
Risk factors
Language:
English
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
Dentistry
Year:
2022
Type:
Article
Affiliation country:
Peru
Institution/Affiliation country:
Universidad Nacional Mayor de San Marcos/PE
/
Universidad Nacional de Trujillo/PE
/
Universidad Peruana Cayetano Heredia/PE
/
Universidad Privada Norbert Wiener/PE
/
Universidad Señor de Sipán/PE
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