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Cornuside ameliorated experimental autoimmune encephalomyelitis by limiting the recruitment of CD4+ T lymphocytes in the spinal cord
Zhang, Rongbo; Zhang, Shuijing; Xu, Bin; Wu, You; Liang, Shunli; Hou, Bonan; Wang, Mimi; Liu, Jin; Yuan, Qiang.
  • Zhang, Rongbo; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Zhang, Shuijing; Zhejiang Rehabilitation Medical Center. Department of Neurological Rehabilitation. Hangzhou. CN
  • Xu, Bin; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Wu, You; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Liang, Shunli; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Hou, Bonan; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Wang, Mimi; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Pharmacy. Hangzhou. CN
  • Liu, Jin; Zhejiang Chinese Medical University. College of Pharmacy. Hangzhou. CN
  • Yuan, Qiang; Zhejiang Chinese Medical University. College of Pharmacy. Hangzhou. CN
Braz. J. Pharm. Sci. (Online) ; 58: e191070, 2022. tab, graf
Article in English | LILACS | ID: biblio-1394044
ABSTRACT
We conducted this study to determine whether cornuside could improve the neurological deficit symptoms of experimental autoimmune encephalomyelitis (EAE) rats, as well as determine the potential involvement of CD4+ T lymphocytes, vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and tumor necrosis factor-α (TNF-α). Altogether, 32 Lewis rats were randomly divided into control, EAE, EAE/prednisolone, and EAE/cornuside, wherein their neurological function was assessed every day. CD4+ T lymphocyte recruitment into the spinal cord (SC) was evaluated using immunohistochemistry. The VCAM-1, ICAM-1 and TNF-α mRNA expressions in the SC were determined by real-time quantitative PCR, and the VCAM-1 and ICAM-1 proteins were determined by western blotting. Compared to the control group, the EAE group rats with neurological deficits had enhanced CD4+ T lymphocyte infiltration and higher expression levels of VCAM-1, ICAM-1, and TNF-α in the SC. Meanwhile, compared with the EAE group, the EAE/cornuside and EAE/prednisolone groups had lower neurological scores, less CD4+ T lymphocyte infiltrations, and lower expression levels of VCAM-1, ICAM-1, and TNF-α in the SC. Thus, cornuside ameliorated EAE, which could be owed to the inhibition of CD4+ T lymphocyte recruitment and VCAM-1, ICAM-1, and TNF-α expressions in the SC
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Full text: Available Index: LILACS (Americas) Main subject: Spinal Cord / CD4-Positive T-Lymphocytes / Encephalomyelitis, Autoimmune, Experimental Limits: Animals Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2022 Type: Article Affiliation country: China Institution/Affiliation country: The Second Affiliated Hospital of Zhejiang Chinese Medical University/CN / Zhejiang Rehabilitation Medical Center/CN

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Full text: Available Index: LILACS (Americas) Main subject: Spinal Cord / CD4-Positive T-Lymphocytes / Encephalomyelitis, Autoimmune, Experimental Limits: Animals Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2022 Type: Article Affiliation country: China Institution/Affiliation country: The Second Affiliated Hospital of Zhejiang Chinese Medical University/CN / Zhejiang Rehabilitation Medical Center/CN