Your browser doesn't support javascript.
loading
Adventitial Collagen Cross-Linking by Glutaraldehyde Reinforcing Human Saphenous Vein - Implication for Coronary Artery Bypass Grafting
Liu, Changcheng; Chen, Duanduan; Li, Zhenfeng; Xu, Huanming; Gu, Chengxiong.
  • Liu, Changcheng; Capital Medical University. Beijing Anzhen Hospital. Department of Cardiac Surgery. Beijing. CN
  • Chen, Duanduan; Beijing Institute of Technology. School of Life Science. Department of Biomedical Engineering. Beijing. CN
  • Li, Zhenfeng; Beijing Institute of Technology. School of Life Science. Department of Biomedical Engineering. Beijing. CN
  • Xu, Huanming; Beijing Institute of Technology. School of Life Science. Department of Biomedical Engineering. Beijing. CN
  • Gu, Chengxiong; Capital Medical University. Beijing Anzhen Hospital. Department of Cardiac Surgery. Beijing. CN
Rev. bras. cir. cardiovasc ; 37(4): 439-446, Jul.-Aug. 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1394715
ABSTRACT
ABSTRACT

Introduction:

A weak venous wall is one of the major reasons contributing to vein graft failure after coronary artery bypass grafting (CABG). We investigated whether adventitial collagen cross-linking by glutaraldehyde reinforces venous wall, preserving the endothelium of veins during high-pressure distention.

Methods:

Human saphenous veins (SVs) were collected from 40 patients undergoing CABG, and adventitia cross-linking was performed with 0.3% glutaraldehyde for five minutes. The cross-linked SVs were accessed by biodegradation assay, immunofluorescent staining, and tensile test. Native SVs and cross-linked SVs from another 20 patients received the 200 mmHg pressure distention for two minutes. Pressure-induced injury of SVs were accessed by immunohistochemistry and electron microscopy.

Results:

Time to digestion was 97±13 minutes for native SVs and 720±0 minutes for cross-linked SVs (P<0.05). After adventitial cross-linking, the collagen I fibres of the vein remarkably presented with compact and nonporous arrangement. In the high-stretch region (stretch ratio 1.4-1.8), the Young's elastic modulus of stress-stretch ratio curve in cross-linked SVs was larger than that in native SVs (13.88 vs. 5.83, P<0.05). The cross-linked SVs had a lower extent of endothelial denudation without fibre fracture during high-pressure distension than native SVs. Comparing with the non-cross-linked SVs, the percentage of endothelial nitric oxide synthase staining length on the endothelium of cross-linked SVs was significantly preserved after high-pressure distension (85.2% vs. 64.7%, P<0.05).

Conclusion:

Adventitial collagen cross-linking by glutaraldehyde reinforced venous wall by increasing stiffness and decreasing extensibility of SVs and mitigated the endothelial damage under high-pressure distension.


Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. bras. cir. cardiovasc Journal subject: Cardiology / General Surgery Year: 2022 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Beijing Institute of Technology/CN / Capital Medical University/CN

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. bras. cir. cardiovasc Journal subject: Cardiology / General Surgery Year: 2022 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Beijing Institute of Technology/CN / Capital Medical University/CN