The use of speckle laser to identify canopy variations in plants subjected to ultra-high diluted substances

ABSTRACT

The reaction of plants to ultra-high dilute substances (UHD) is well known, however, the signaling of the immediate effect still doesn't have a widely accepted methodology. The objective of this experiment was to use non-destructive sampling to find signs of UHD soon after application to plants. Methods:The control consisted of untreated purslane [Pilea microphylla (L.) Liebm] plants and imaged with a digital cameraMobius (CMOS 1270x720 pixels) directed at a laser beam (±680 nm) emitted over the plant canopy for 220 seconds, with 6-second intervals. Then, the same plants were treated with Fluoricum acidum30CH (Fl. ac.30),and ten minutes later, new images of the leaves were taken to verify the possible existence of reaction patterns of the plants generated by Biospeckle Laser (1,2). Results:Several types of imaging were performed to choose the image pattern, and the NIR type was chosen, generated by the Mobius camera connected directly to a laptop (Figure 1). The images were treated using the THSP algorithm, which generated data to compare the variation of pixel intensity with and without the presence of UHD. Conclusion:Research has shown that "Fl. ac. 30" is identified in purslane plants soon after application and this sign persists for at least 180 minutesafter application, with a significant difference from the control at the 1% probability level.