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Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection
Zhang, Mei; Du, Yanhua; Yang, Li; Zhan, Lin; Yang, Bin; Huang, Xueyong; Xu, Bianli; Morita, Koichi; Yu, Fuxun.
  • Zhang, Mei; Guizhou University. School of Medicine. Guiyang. CN
  • Du, Yanhua; Center for Disease Control and Prevention of Shanxi Province. Xian. CN
  • Yang, Li; Guizhou University. School of Medicine. Guiyang. CN
  • Zhan, Lin; Guizhou Provincial Peoples Hospital. Guiyang. CN
  • Yang, Bin; Guizhou Provincial Peoples Hospital. Guiyang. CN
  • Huang, Xueyong; Center for Disease Control and Prevention of Henan Province. Zhengzhou. CN
  • Xu, Bianli; Center for Disease Control and Prevention of Henan Province. Zhengzhou. CN
  • Morita, Koichi; Nagasaki University. Institute of Tropical Medicine. Department of Virology. Nagasaki. JP
  • Yu, Fuxun; Guizhou Provincial Peoples Hospital. Guiyang. CN
Braz. j. infect. dis ; 26(4): 102386, 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1403881
ABSTRACT
ABSTRACT

Introduction:

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly emerged virus that poses a great threat to human health because of high fatality rate.

Methods:

To develop sensitive and specific sero-diagnostic systems for SFTSV infections, monoclonal antibodies (MAbs) against recombinant SFTSV nucleocapsid (rSFTSV-N) protein were developed by immunizing BALB/C mice with rSFTSV-N protein and fusing the spleen cells with SP2/0 myeloma cells. Three hybridoma cell lines secreting MAbs against rSFTSV-N were obtained. MAb based IgG sandwich enzyme linked immunosorbent assay (ELISA) and IgM capture ELISA systems were established by using the newly developed MAbs. One hundred fifteen clinical suspected SFTS patients serum samples were used to evaluate the newly established systems by comparing with the total antibody detecting sandwich ELISA system and indirect ELISA systems.

Results:

The MAbs based sandwich IgG ELISA was perfectly matched with that of the total antibody sandwich ELISA and the indirect IgG ELISA. IgM capture ELISA results perfectly matched with that of the total antibody sandwich ELISA while detecting eight additional positive samples missed by the indirect IgM ELISA.

Conclusions:

The MAbs against rSFTSV-N protein offer new tools for SFTSV studies and our newly developed MAb-based IgG and IgM capture ELISA systems would offer safe and useful tools for diagnosis of SFTS virus infections and epidemiological investigations.


Full text: Available Index: LILACS (Americas) Type of study: Diagnostic study Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2022 Type: Article / Project document Affiliation country: China / Japan Institution/Affiliation country: Center for Disease Control and Prevention of Henan Province/CN / Center for Disease Control and Prevention of Shanxi Province/CN / Guizhou Provincial Peoples Hospital/CN / Guizhou University/CN / Nagasaki University/JP

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Full text: Available Index: LILACS (Americas) Type of study: Diagnostic study Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2022 Type: Article / Project document Affiliation country: China / Japan Institution/Affiliation country: Center for Disease Control and Prevention of Henan Province/CN / Center for Disease Control and Prevention of Shanxi Province/CN / Guizhou Provincial Peoples Hospital/CN / Guizhou University/CN / Nagasaki University/JP