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Extended-spectrum Beta-lactamases Encoding Genes among Salmonella Enterica serovar Typhi Isolates in Patients with Typhoid Fever from four Academic Medical Centers in Lagos, Nigeria
Akinyemi, Kabiru O; Al-Khafaji, Noor S.K.; Al-Alaq, Farah T.; Fakorede, Christopher O; Al-Dahmoshi, Hussein O.M.; Iwalokun, Bamidele A; Akpabio, Imaobong; Alkafaas, Samar Sami; Saki, Morteza.
  • Akinyemi, Kabiru O; Lagos State University. Department of Microbiology. Faculty of Science. Ojo. NG
  • Al-Khafaji, Noor S.K.; University of Babylon. Department of Biology. College of Science. Babylon, Hilla City. IQ
  • Al-Alaq, Farah T.; University of Babylon. Department of Biology. College of Science. Babylon, Hilla City. IQ
  • Fakorede, Christopher O; Lagos State University. Department of Microbiology. Faculty of Science. Ojo. NG
  • Al-Dahmoshi, Hussein O.M.; University of Babylon. Department of Biology. College of Science. Babylon, Hilla City. IQ
  • Iwalokun, Bamidele A; Nigerian Institute of Medical Research. Department of Molecular Biology and Biotechnology. Yaba. NG
  • Akpabio, Imaobong; Lagos State University. Department of Microbiology. Faculty of Science. Ojo. NG
  • Alkafaas, Samar Sami; Tanta University. Faculty of Science. Tanta. EG
  • Saki, Morteza; Ahvaz Jundishapur University of Medical Sciences. Department of Microbiology. Faculty of Medicine. Ahvaz. IR
Rev. invest. clín ; 74(3): 165-171, May.-Jun. 2022. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1409575
ABSTRACT
ABSTRACT

Background:

There is scarce information about the occurrence of extended-spectrum β-lactamases (ESBLs) in Salmonella enterica serovar Typhi (S. Typhi) from patients with typhoid fever.

Objective:

To study the antimicrobial resistance and ESBL encoding genes among S. Typhi isolates in aforesaid patients from Lagos, Nigeria.

Methods:

S. Typhi isolates were collected from blood samples of typhoid fever patients from 4 academic medical centers in Lagos, Nigeria. The identification of isolates and their antibiotic susceptibility testing were performed by standard bacteriological techniques and disc diffusion method, respectively. The production of ESBLs was investigated using combination disk test (CDT) and polymerase chain reaction (PCR).

Results:

A total of 27 S. Typhi isolates was collected. All isolates were susceptible to imipenem and nitrofurantoin. Fifteen (55.6%) isolates were multidrug-resistant (MDR). The CDT test showed 11 (40.7%) ESBL producer isolates. However, the PCR revealed a higher occurrence rate for ESBL producers (66.7%, n = 18/27). The ESBL genes were as follows blaCTX-M (37.0%, n = 10/27), blaSHV (18.5%, n = 5/27), and blaTEM (44.4%, n = 12/27). All ESBL positive S. Typhi isolates were MDR.

Conclusions:

This study showed the emergence of ESBL-harboring S. Typhi in patients with typhoid fever from Nigeria.


Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. invest. clín Journal subject: Medicine Year: 2022 Type: Article Affiliation country: Egypt / Iran / Iraq / Nigeria Institution/Affiliation country: Ahvaz Jundishapur University of Medical Sciences/IR / Lagos State University/NG / Nigerian Institute of Medical Research/NG / Tanta University/EG / University of Babylon/IQ

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Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. invest. clín Journal subject: Medicine Year: 2022 Type: Article Affiliation country: Egypt / Iran / Iraq / Nigeria Institution/Affiliation country: Ahvaz Jundishapur University of Medical Sciences/IR / Lagos State University/NG / Nigerian Institute of Medical Research/NG / Tanta University/EG / University of Babylon/IQ