Molecular detection of anaplasmataceae agents on dogs from the departament of Piura, Peru
Rev. patol. trop
;
51(2): 134-144, 2022. ilus
Article
in English
| LILACS
| ID: biblio-1414365
ABSTRACT
This study aimed to study the prevalence of Anaplasmataceae organisms through the nested-PCR and phylogenetic analysis on domestic dogs in the Department of Piura, Peru. Two hundred and twelve canine blood samples were randomly collected on dogs from the central urban areas at the Piura Department in Peru. The extracted DNAs were tested, by nested-PCR based on 16SrRNA gene, to identify agents from Anaplasmataceae family. These results show that there was a prevalence of 18.5% (40/216) of positive dogs, 13.8% (30/216) for Ehrlichia canis, 7.4% (16/216) for Anaplasma platys and 0.1% (2/216) for Ehrlichia sp. confirmed by sequencing analysis. Co-positivity among Anaplasmataceae family species was present in 25% (10/40) of positive samples. There was a significant association among Anaplasmataceae family infection in dogs and the following variables sex (p=0.034), presence of ticks (p=0.0001), and socio-economic status (p=0.001). There was no statistical association on the variables "living with other animals" and "age group" (p=0.1074). The partial sequences on the portion of the 16S rRNA gene, from positive samples for agents of Anaplasmataceae family demonstrated an identity of 97-100% with the isolated E. canis and A. platys obtained from the GenBank. This is the first study on infection by agents of Anaplasmataceae family in dogs in the Department of Piura, through molecular analysis:
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Subject(s)
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Ticks
/
Polymerase Chain Reaction
/
Urban Area
/
Ehrlichia canis
/
Anaplasma
/
Anaplasmataceae
Type of study:
Diagnostic study
/
Prognostic study
/
Risk factors
Country/Region as subject:
South America
/
Peru
Language:
English
Journal:
Rev. patol. trop
Journal subject:
Tropical Medicine
/
Pathology
Year:
2022
Type:
Article
Affiliation country:
Brazil
/
Peru
Institution/Affiliation country:
Universidad Nacional Mayor de San Marcos/PE
/
Universidade Federal Fluminense/BR
/
Universidade Federal Rural do Rio de Janeiro/BR
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