Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
Biosci. j. (Online)
;
39: e39019, 2023. ilus, graf, tab
Article
in English
| LILACS
| ID: biblio-1415913
ABSTRACT
Acinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.0028. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent.
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Subject(s)
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cross Infection
/
Acinetobacter baumannii
Type of study:
Observational study
/
Prevalence study
/
Risk factors
Language:
English
Journal:
Biosci. j. (Online)
Journal subject:
Agricultura
/
Disciplinas das Cincias Biol¢gicas
/
Pesquisa Interdisciplinar
Year:
2023
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro Universitário Atenas/BR
/
Centro Universitário de Mineiros/BR
/
Universidade Federal de Uberlândia/BR
/
Universidade Federal do Triângulo Mineiro/BR
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