Salmonella Heidelberg and Salmonella Minnesota in Brazilian broilers: Genomic characterization of third-generation cephalosporin and fluoroquinolone-resistant strains
Environ Microbiol Rep
;
x2023.
Article
in English
| LILACS, CONASS, ColecionaSUS, SES-SP, SESSP-IALPROD, SES-SP
| ID: biblio-1417271
ABSTRACT
Salmonella serovars Heidelberg and Minnesota encoding antimicrobial resistance to third-generation cephalosporins and fluoroquinolones are often detected in poultry/poultry meat. We analysed the genomes of 10 Salmonella Heidelberg (SH) and 4 Salmonella Minnesota (SM) from faecal isolates of Brazilian poultry. These featured virulent and multidrug-resistant characteristics, with AmpC beta-lactamase (blaCMY-2 ) predominance (9/14), for all SM (4/4) and some SH (3/10) located on IncC plasmid replicons. IncC carrying blaCTX-M-2 was only detected among SH (3/10). Mutation in the gyrA/parC genes was present in all SH, whereas SM harboured parC mutation plus qnrB19 on ColRNAI plasmids (3/4). In silico resistance overall corroborated with phenotypic results. Core genome phylogenies showed close clustering and high similarities between the Brazilian and poultry meat/food isolates from Europe, and to human isolates from European countries with documented import of Brazilian poultry meat. Conjugation assays with SM successfully transferred blaCMY-2 , and qnrB19 to an Escherichia coli recipient. The findings reinforce the ongoing antimicrobial resistance acquisition of SH and Minnesota and the risks for disseminating resistant strains and/or mobile elements which may increasingly affect importing countries and the need for controlling AMR in major poultry-exporting countries like Brazil.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Salmonella
/
Sprains and Strains
/
Cephalosporins
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Environ Microbiol Rep
Year:
2023
Type:
Article
Institution/Affiliation country:
Bacteriology Center, Adolfo Lutz Institute/BR
/
Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen/DK
/
Department of Clinical Analysis, School of Pharmaceutical Sciences, University of São Paulo/BR
/
Department of Microbiology, Institute of Biomedical Science, University of São Paulo/BR
/
Department of Veterinary Pathology, School of Veterinary Medicine and Animal Science/BR
/
Department of Veterinary Pathology, School of Veterinary Medicine and Animal Science,/BR
/
Department of Veterinary and Animal Sciences, Section for Food Safety and Zoonoses/BR
/
Department of Veterinary and Animal Sciences, Section for Food Safety and Zoonoses, Institute for Veterinary and Companion Animal Science, Københavns Universitet, Copenhagen, Denmark/BR
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