MicroRNA-375 inhibits laryngeal squamous cell carcinoma progression via targeting CST1
Braz. j. otorhinolaryngol. (Impr.)
;
88(supl.4): S108-S116, Nov.-Dec. 2022. tab, graf
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1420879
ABSTRACT
Abstract Objective:
This study aims to explore the effect and mechanism of miR-375 in Laryngeal Squamous Cell Carcinoma (LSCC) cell progression.Methods:
LSCC cells (LSC-1 and TU177) were transfected with miR-375-mimic, miR-375-inhibitor or miR-375-mimic + oe-CST1. The expression of miR-375, CST1, MMP-2, and MMP-9 was measured. The effect of miR-375-mimic, miR-375-inhibitor or miR-375-mimic + oe-CST1 on cell biological functions, including cell proliferation, migration, invasion, and apoptosis, was also assessed. The potential relationship between CST1 and miR-375 was predicted by Jefferson software and validated by dual luciferase reporter gene assay.Results:
Downregulated miR-375 expression was found in LSCC cells. Overexpression of miR-375 inhibited the viability and migration and promoted apoptosis of LSCC cells. Jefferson database and dual luciferase reporter gene assay confirmed that miR-375 directly targeted CST1. Over-expression of CST1 could reverse the anti-cancer effect of miR-375 overexpression in LSCC cells.Conclusion:
Collected evidence showed that miR-375/CST1 axis was implicated in LSCC progression. Level of evidence Level 3
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Braz. j. otorhinolaryngol. (Impr.)
Journal subject:
Otolaryngology
Year:
2022
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Jiangxi Pingxiang Peoples Hospital/CN
Similar
MEDLINE
...
LILACS
LIS