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C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways
CHEN, Yan; LIU, Yang; LI, Hao; HUNA, Risu; TAN, Xiaohan; LI, Ning; ZHANG, Yiying; JIAO, Xiaohui; LIU, Mingyue.
  • CHEN, Yan; Harbin Medical University. Department of Oral Maxillofacial Surgery. The First Affiliated Hospital. Harbin. CN
  • LIU, Yang; Heilongjiang Provincial Hospital. Department of Stomatology. Harbin. CN
  • LI, Hao; Heilongjiang University of Traditional Chinese Medicine. Department of Stomatology. The Fourth Affiliated Hospital. Harbin. CN
  • HUNA, Risu; Harbin Medical University. Oral Implant Center. The Second Affiliated Hospital. Harbin. CN
  • TAN, Xiaohan; Harbin Medical University. Department of Prosthodontics. The Second Affiliated Hospital. Harbin. CN
  • LI, Ning; Harbin Medical University. Department of Cardiology. The Second Affiliated Hospital. Harbin. CN
  • ZHANG, Yiying; Harbin Medical University. Oral Implant Center. The Second Affiliated Hospital. Harbin. CN
  • JIAO, Xiaohui; Harbin Medical University. Department of Oral Maxillofacial Surgery. The First Affiliated Hospital. Harbin. CN
  • LIU, Mingyue; Harbin Medical University. Department of Prosthodontics. The Second Affiliated Hospital. Harbin. CN
J. appl. oral sci ; 31: e20220404, 2023. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1421902
ABSTRACT
Abstract Objective Abnormal complement activation is associated with periodontitis. W54011 is a novel non-peptide C5aR antagonist (C5aRA) that exhibits favorable anti-inflammatory effects in various inflammatory models. However, whether W54011 inhibits periodontitis has not yet been fully elucidated. To address this, we have investigated the probable anti-inflammatory mechanism of W54011 in LPS-treated inflammation in human gingival fibroblasts (HGFs). Methodology HGFs were isolated from healthy gingival tissue samples using the tissue block method and were identified with immunofluorescence staining. The CCK8 assay and reverse transcription-PCR (RT-PCR) were used to select the optimal induction conditions for Lipopolysaccharide (LPS) and C5aRA (according to supplementary data S1, S2 and S3). The levels of inflammatory cytokines, C5aR, and the activation of NF-κB/MAPK signaling pathways were determined by RT-quantitative PCR (RT-qPCR) and Western blotting. Results Immunofluorescence results showed that vimentin and FSP-1 were positive in HGFs and Keratin was negative in HGFs. Immunofluorescence staining demonstrated that C5aRA inhibited LPS-stimulated nuclear translocation of p-p65. RT-qPCR and Western blotting showed that C5aRA reduced the expression of IL-1β, IL-6, TNF-α, C5aR, p-p65, p-IκBα, p-JNK, p-c-JUN, and TLR4 in LPS-induced HGFs. Conclusion These findings suggested that C5aRA attenuated the release of inflammatory cytokines in LPS-induced HGFs by blocking the activation of the NF-κB and MAPK signaling pathways.


Full text: Available Index: LILACS (Americas) Type of study: Prognostic study Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2023 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Harbin Medical University/CN / Heilongjiang Provincial Hospital/CN / Heilongjiang University of Traditional Chinese Medicine/CN

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Full text: Available Index: LILACS (Americas) Type of study: Prognostic study Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2023 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Harbin Medical University/CN / Heilongjiang Provincial Hospital/CN / Heilongjiang University of Traditional Chinese Medicine/CN