Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus
Rev. Soc. Bras. Med. Trop
;
56: e0217, 2023. graf
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1422879
ABSTRACT
ABSTRACT Background:
Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR.Methods:
A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique.Results:
Three specific primers with 100% specificity for the Sporothrix genus were generated.Conclusions:
PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2023
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Hospital Santa Casa de Belo Horizonte/BR
/
Universidade Federal de Minas Gerais/BR
/
Universidade Federal de Ouro Preto/BR
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