Investigation of the histopathological level of Ki-67, caspase-3 expressions of the effects of hesperidin on wound healing in the rat esophagus
Acta cir. bras
;
38: e381723, 2023. tab, ilus
Article
in English
| LILACS, VETINDEX
| ID: biblio-1429536
ABSTRACT
Purpose:
The effects of hesperidin application on the wound caused by esophageal burns were investigated in this study.Methods:
Wistar albino rats were divided into three groups Control group only 1 mL of 0.09% NaCl was administered i.p. for 28 days; Burn group An alkaline esophageal burn model was created with 0.2 mL of 25% NaOH orally by gavage1 mL of 0.09% NaCl was administered i.p. for 28 days; Burn+Hesperidin group 1 mL of 50 mL/kg of hesperidin was given i.p. for 28 days to rats after burn injury. Blood samples were collected for biochemical analysis. Esophagus samples were processed for histochemical staining and immunohistochemistry.Results:
Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were significantly increased in Burn group. Glutathione (GSH) content and histological scores of epithelialization, collagen formation, neovascularization was decreased. After hesperidin treatment, these values were significantly improved in the Burn+Hesperidin group. In the Burn group, epithelial cells and muscular layers were degenerated. Hesperidin treatment restored these pathologies in Burn+Hesperidin group. Ki-67 and caspase-3 expressions were mainly negative in control group; however, the expression was increased in the Burn group. In the Burn+Hesperidin group, Ki-67 and caspase-3 immune activities were reduced.Conclusion:
Hesperidin dosage and application methods can be developed as an alternative treatment for burn healing and treatment.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Wound Healing
/
Apoptosis
/
Ki-67 Antigen
/
Esophagus
/
Caspase 3
/
Hesperidin
Type of study:
Prognostic study
Language:
English
Journal:
Acta cir. bras
Year:
2023
Type:
Article
Institution/Affiliation country:
Dicle Üniversitesi/TR
/
Diyarbakir Memorial Hospital/TR
/
Siirt University/TR
Similar
MEDLINE
...
LILACS
LIS