Effects of Chitosan Nanoparticles with Long Synthetic siRNAs Targeting VEGF in Triple-Negative Breast Cancer Cells
Braz. J. Pharm. Sci. (Online)
;
59: e22304, 2023. tab, graf
Article
in English
| LILACS
| ID: biblio-1447564
ABSTRACT
Abstract Vascular endothelial growth factor (VEGF) is an essential angiogenic factor in breast cancer development and metastasis. Small interfering RNAs (siRNAs) can specifically silence genes via the RNA interference pathway, therefore were investigated as cancer therapeutics. In this study, we investigated the effects of siRNAs longer than 30 base pairs (bp) loaded into chitosan nanoparticles in triple-negative breast cancer cells, compared with conventional siRNAs. 35 bp long synthetic siRNAs inhibited VEGF gene expression by 51.2% and increased apoptosis level by 1.75-fold in MDA-MB-231 cell lines. Furthermore, blank and siRNA-loaded chitosan nanoparticles induced expression of IFN-γ in breast cancer cells. These results suggest that long synthetic siRNAs can be as effective as conventional siRNAs, when introduced into cells with chitosan nanoparticles
Full text:
Available
Index:
LILACS (Americas)
Main subject:
RNA, Small Interfering
/
Vascular Endothelial Growth Factor A
/
Chitosan
/
Nanoparticles
/
Triple Negative Breast Neoplasms
Type of study:
Diagnostic study
Language:
English
Journal:
Braz. J. Pharm. Sci. (Online)
Journal subject:
Farmacologia
/
Teraputica
/
Toxicologia
Year:
2023
Type:
Article
Affiliation country:
Turkey
Institution/Affiliation country:
Istanbul Medipol University/TR
/
Marmara University/TR
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