Electroacupuncture ameliorates experimental colitis induced by TNBS through activation of interleukin-10 and inhibition of iNOS in mice
Acta cir. bras
;
29(12): 1-7, 12/2014. graf, tab, ilus
Article
in English
| LILACS, VETINDEX
| ID: biblio-1456222
ABSTRACT
PURPOSE:
To study the anti-inflammatory actions of electroacupuncture (EAc) on an experimental colitis model in mice.METHODS:
Thirty-eight male Swiss mice, divided in five groups, were subjected to induction of colitis by TNBS in 50% ethanol. Saline (SAL) and ethanol (ETNL) groups served as controls. TNBS+EAc and TNBS+ dexamethasone subgroups were treated with EAc 100Hz and dexamethasone (DEXA) 1 mg/Kg/day, respectively. After three days, a colon segment was obtained for quantification of myeloperoxidase (MPO) activity, immunohistochemistry for iNOS, malondialdehyde (MDA) and cytokines (IL-1β and IL-10).RESULTS:
Neutrophilic activity, assayed as MPO activity, was significantly higher in the TNBS colitis group than that in the saline control group. TNBS+EAc group showed suppression of IL-10 in the colon. EAc treatment significantly reduced the concentration of MDA and the expression of iNOS, as compared to the other groups.CONCLUSION:
Electroacupuncture 100Hz applied to acupoint ST-36 promotes an anti-inflammatory action on the TNBS-induced colitis, mediated by increase of IL-10 and decrease of iNOS expression.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trinitrobenzenes
/
Electroacupuncture
/
Colitis
/
Nitric Oxide Synthase
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Year:
2014
Type:
Article
Institution/Affiliation country:
Federal University of Ceara (UFC)/BR
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