Stereological study and analysis of oxidative stress during renal aging in rats
Acta cir. bras
;
35(11): e351103, 2020. ilus, tab, graf
Article
in English
| LILACS, VETINDEX
| ID: biblio-1456240
ABSTRACT
Purpose To evaluate renal histological changes by stereology and morphometry and analyze the main markers of oxidative stress in rats undergoing natural aging. Methods Seventy two Wistar rats were divided into six groups of 12 rats each, which were euthanized at 3, 6, 9, 12, 18, and 24 months of age. Right kidney was stereologically and morphometrically analyzed to calculate the volumetric density (Vv[glom]), numerical density (Nv[glom]) and glomerular volume (Vol[glom]). Left kidney was used to determine the levels of nonprotein thiols, lipid peroxidation, and protein carbonylation, as well as the activities of superoxide-dismutase and catalase enzymes. Results Both Vv[glom] and Nv[glom] values showed gradual decreases between groups. Activity of superoxide-dismutase was elevated at 24 months of age, and the levels of nonprotein thiols were higher in older animals. Greater catalase activity and protein carbonylation were observed in animals between 6 and 12 months of age but lessened in older rats. Lipid peroxidation decreased in the older groups. Conclusions Morphometric and stereological analyses revealed a gradual decrease in the volume and density of renal glomeruli during aging, as well as kidney atrophy. These findings related to oxidative stress clarify many changes occurring in kidney tissues during senescence in rats.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Aging
/
Oxidative Stress
/
Kidney Diseases
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Year:
2020
Type:
Article
Institution/Affiliation country:
Universidade Federal do Paraná/BR
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