Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium(Cambess) O. Berg
Acta sci., Biol. sci
;
41: e46358, 20190000. graf, ilus
Article
in English
| LILACS, VETINDEX
| ID: biblio-1460879
ABSTRACT
Campomanesia adamantiumis a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA, NAA or picloram. Cytochemical and SEM analyses revealed cellular characteristics of the formation of meristematic centers that indicated 4.14 μM of picloram to be the best treatment for induction of embryogenic calli, and demonstrating their embryogenic potential. The treatment was used to establish a callus growth curve, from which it was inferred that calli should be transferred to new culture media on the 28thday to maintain cell viability.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Myrtaceae
/
Embryonic Development
Language:
English
Journal:
Acta sci., Biol. sci
Year:
2019
Type:
Article
Institution/Affiliation country:
Universidade Federal de Jataí/BR
/
Universidade Federal de Lavras/BR
/
Universidade de São João Del-Rei/BR
Similar
MEDLINE
...
LILACS
LIS