In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations
Acta sci., Biol. sci
;
43: e52866, 2021. tab
Article
in English
| LILACS, VETINDEX
| ID: biblio-1460986
ABSTRACT
In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
In Vitro Techniques
/
Manihot
/
Naphthaleneacetic Acids
Type of study:
Practice guideline
Language:
English
Journal:
Acta sci., Biol. sci
Year:
2021
Type:
Article
Institution/Affiliation country:
Empresa Brasileira de Pesquisa Agropecuária/BR
/
Faculdade Maria Milza/BR
/
Universidade Federal do Recôncavo da Bahia/BR
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