Comparison of phenotypic and molecular tests to identify lactic acid bacteria

Mendonça Moraes, Paula; Martins Perin, Luana; Silva Júnior, Abelardo; Augusto Nero, Luís

Mendonça Moraes, Paula; Martins Perin, Luana; Silva Júnior, Abelardo; Augusto Nero, Luís.

Mendonça Moraes, Paula; Universidade Federal de Viçosa Departamento de Veterinária.
Martins Perin, Luana; Universidade Federal de Viçosa Departamento de Veterinária.
Silva Júnior, Abelardo; Universidade Federal de Viçosa Departamento de Veterinária.
Augusto Nero, Luís; Universidade Federal de Viçosa Departamento de Veterinária.

Braz. j. microbiol ; 44(1)2013.

Article in English | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469584

ABSTRACT

ABSTRACT

Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.

16sDNA; API; Biolog; Identification; Lactic acid bacteria; PCR

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Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. j. microbiol Year: 2013 Type: Article