It is time for top-down venomics
J. venom. anim. toxins incl. trop. dis
;
232017.
Article
in English
|
LILACS-Express
| LILACS, VETINDEX
| ID: biblio-1484700
ABSTRACT
Abstract The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (TDP) analyzes intact proteins without digestion and provides high quality data to identify and characterize toxiforms. Denaturing top-down proteomics is the most disseminated subarea of TDP, which performs qualitative and quantitative analyzes of proteoforms up to ~30 kDa in high-throughput and automated fashion. On the other hand, native top-down proteomics provides access to information on large proteins (> 50 kDA) and protein interactions preserving non-covalent bonds and physiological complex stoichiometry. The use of native and denaturing top-down venomics introduced novel and useful techniques to toxinology, allowing an unprecedented characterization of venom proteins and protein complexes at the toxiform level. The collected data contribute to a deep understanding of venom natural history, open new possibilities to study the toxin evolution, and help in the development of better biotherapeutics.
Full text:
Available
Index:
LILACS (Americas)
Type of study:
Prognostic study
/
Qualitative research
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Year:
2017
Type:
Article
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