A rat model of dual-flow liver machine perfusion system
Acta cir. bras
;
38: e387723, 2023. graf, ilus
Article
in English
| LILACS, VETINDEX
| ID: biblio-1519877
ABSTRACT
Purpose:
As clinical liver perfusion systems use portal vein and artery flow, dual perfusion techniques are required even in small animal models in order to reproduce clinical setting. The aim of this study was to construct a new dual-flow perfusion system in rat model and optimized the oxygen supply to ensure the aerobic metabolization.Methods:
The dual-flow circuit was fabricated using rat liver and whole blood samples as perfusates. The oxygen supply was controlled according to the amount of dissolved oxygen in the perfusate. Perfusate parameters and adenosine triphosphate (ATP) levels were analyzed to evaluate organ function and metabolic energy state. Stored whole blood also tested the suitability as perfusate.Results:
Stored blood showed decrease oxygen delivery and liver function compared to fresh blood. Using fresh blood as perfusate with air only, the dissolved oxygen levels remained low and anaerobic metabolism increased. In contrast, with oxygen control at living body level, anaerobic metabolism was well suppressed, and tissue ATP content was increased.Conclusions:
We developed a new dual-flow system that enable to reproduce the clinical settings. The perfusion system showed the possibility to improve the energy metabolic state of the perfused organ under appropriate partial pressure of oxygen.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Perfusion
/
Oxygenation
/
Ischemia
/
Liver
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2023
Type:
Article
Affiliation country:
Japan
Institution/Affiliation country:
Nippon Veterinary and Life Science University/JP
/
Screen Holdings Co., Ltd/JP
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