Sevoflurane attenuates myocardial ischemia/reperfusion injury by up-regulating microRNA-99a and down-regulating BRD4
Acta cir. bras
;
38: e383123, 2023. graf
Article
in English
| LILACS, VETINDEX
| ID: biblio-1519885
ABSTRACT
Purpose:
It has been explored that sevoflurane (Sevo) is cardioprotective in myocardial ischemia/reperfusion injury (MI/RI) and mediates microRNA (miRNA) expression that control various physiological systems. Enlightened by that, the work was programmed to decode the mechanism of Sevo and miR-99a with the participation of bromodomain-containing protein 4 (BRD4).Methods:
MI/RImodel was established on mice. MI/RI modeled mice were exposed to Sevo or injected with miR-99a or BRD4-related vectors to identify their functions in cardiac function, pathological injury, cardiomyocyte apoptosis, inflammation, and oxidative stress in MI/RI mice. MiR-99a and BRD4 expression in myocardial tissues were tested, and their relation was further validated.Results:
MiR-99a was down-regulated, and BRD4 was up-regulated in MI/RI mice. Sevo up-regulated miR-99a to inhibit BRD4 expression in myocardial tissues of MI/RI mice. Sevo improved cardiac function, relieved myocardial injury, repressed cardiomyocyte apoptosis, and alleviated inflammation and oxidative stress in mice with MI/RI. MiR-99a restoration further enhanced the positive effects of Sevo on mice with MI/RI. Overexpression of BRD4 reversed up-regulation of miR-99a-induced attenuation of MI/RI in mice.Conclusions:
The work delineated that Sevo up-regulates miR-99a to attenuate MI/RI by inhibiting BRD4.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Reperfusion Injury
/
Myocardial Ischemia
/
Sevoflurane
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2023
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Wuhan No 1 Hospital/CN
Similar
MEDLINE
...
LILACS
LIS