Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms
Braz. j. oral sci
;
22: e231137, Jan.-Dec. 2023. ilus
Article
in English
| LILACS, BBO
| ID: biblio-1523140
ABSTRACT
The purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods:
F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05).Results:
Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose.Conclusions:
Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharidesSubject(s)
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Polysaccharides, Bacterial
/
Fusobacterium nucleatum
/
Biofilms
/
Nicotine
Limits:
Humans
Language:
English
Journal:
Braz. j. oral sci
Journal subject:
Dentistry
Year:
2023
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Indiana University/US
/
University of Campinas/BR
/
University of Detroit Mercy/US
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