Maturation and development of fetal pig intestinal tissue in immunodeficient mice
Acta cir. bras
;
39: e390624, 2024. ilus
Article
in English
| LILACS, VETINDEX
| ID: biblio-1533361
ABSTRACT
Purpose:
This study aimed to compare the degree of maturation and development of fetal pig segmental intestinal tissue with that of spheroids created by in-vitro reaggregation of dissociated fetal intestinal cells after transplantation into immunodeficient mice.Methods:
Fetal pig small intestines were transplanted as segmental grafts into the omentum and subrenal capsules of immunodeficient mice or enzymatically treated to generate single cells. Spheroids made by in-vitro reaggregation of these cells were transplanted into the subrenal capsules of immunodeficient mice. The segmental grafts and spheroids were harvested four and eight weeks after transplantation, and the structural maturity and in-vivo development of these specimens were histologically evaluated.Results:
The spheroids were engrafted and supplied blood vessels from the host mice, but an intestinal layered structure was not clearly observed, and there was almost no change in size. On the other hand, the segmental grafts formed deep crypts in the mucus membrane, the inner circular layer, and outer longitudinal muscles. The crypts of the transplanted grafts harvested at eight weeks were much deeper, and the smooth muscle layer and the enteric nervous system were more mature than those of grafts harvested at the fourth week, although the intestinal peristaltic wave was not observed.Conclusions:
Spheroids created from fetal small intestinal cells could not form layered structures or mature sufficiently. Conversely, segmental tissues structurally matured and developed after in-vivo transplantation and are therefore potential grafts for transplantation.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Swine
/
Transplantation, Heterologous
/
Fetal Tissue Transplantation
/
Fetal Organ Maturity
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2024
Type:
Article
Affiliation country:
Japan
Institution/Affiliation country:
Jikei University School of Medicine/JP
/
The Jikei University School of Medicine/JP
/
The University of Tokyo/JP
Similar
MEDLINE
...
LILACS
LIS