The influence of growth time on the identification of Bartonella henselae strains by MALDI-TOF mass spectrometry
Rev. Inst. Med. Trop. São Paulo (Online)
;
66: e9, 2024. tab
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1535309
ABSTRACT
ABSTRACT Bartonella spp. are bacteria responsible for neglected diseases worldwide. Bartonella henselae is the species most associated with human infections. It is associated with a large spectrum of clinical manifestations and is potentially fatal. The identification of Bartonella spp. is considered a challenge in clinical routine. These bacteria are fastidious, and the time required to isolate them varies from one to six weeks. MALDI-TOF mass spectrometry has emerged as an application for research on Bartonella spp. , and has still been little explored. We investigated whether three different B. henselae strains with different growth times—14 and 28 days—could be correctly identified by MALDI-TOF mass spectra fingerprint comparison and matching. We found that the spectra from strains with different growth times do not match each other, leading to misidentification. We suggest creating database entries with multiple spectra from strains with different growth times to increase the chances of accurate identification of Bartonella spp. by MALD-TOF MS.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Rev. Inst. Med. Trop. São Paulo (Online)
Journal subject:
Medicina Tropical
Year:
2024
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Campinas/BR
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