Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice
Acta cir. bras
;
31(10): 675-679, Oct. 2016. graf
Article
in English
| LILACS
| ID: biblio-827651
ABSTRACT
ABSTRACT PURPOSE:
To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model.METHODS:
Two groups were constituted The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups Tx, IPC-R, Fk, IPC-Fk, in both strains. Intestinal grafts were stained with hematoxylin and eosin and immunohistochemically.RESULTS:
The graft development evaluation in ISO group showed that IPC-R reduced the development compared with ISO-Tx (5.2±0.4 vs 9.0±0.8) and IPC-R-Fk increased the graft development compared with IPC-R (11.2±0.7 and 10.2±0.8). In ALO group, IPC-Fk increased the development compared with ALO-Tx and ALO with IPC-R (6.0±0.8, 9.0±1.2, 0.0±0.0, 0.5±0.3). The PCNA expression was increased in ISO group treated with Fk and IPC-R compared to other groups (12.2±0.8 vs Tx 8.8±0.9, IPC-R 8.0±0.4 and Fk 9.0±0.6). The graft rejection was lower in groups treated with IPC-R (-18%), Fk (-68%) or both (-61%) compared with ALO-Tx.CONCLUSION:
Remote ischemic preconditioning showed benefic effect even associate with Tacrolimus on the development and acute rejection of the fetal small bowel graft in the Isogenic and Allogenic transplants.
Full text:
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Index:
LILACS (Americas)
Main subject:
Fetal Tissue Transplantation
/
Tacrolimus
/
Ischemic Preconditioning
/
Immunosuppressive Agents
/
Intestine, Small
Type of study:
Evaluation studies
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2016
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de São Paulo/BR
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