Thermostable chitinase from Cohnella sp. A01: isolation and product optimization
Braz. j. microbiol
;
47(4): 931-940, Oct.-Dec. 2016. tab, graf
Article
in English
| LILACS
| ID: biblio-828205
ABSTRACT
Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and temperature of incubation were the most significant factors influencing chitinase production. From the tested values, the best pH/temperature was obtained at pH 5 and 70 °C, with Km and V max values of chitinase to be 5.6 mg/mL and 0.87 µmol/min, respectively. Ag+, Co2+, iodoacetamide, and iodoacetic acid inhibited the enzyme activity, whereas Mn2+, Cu2+, Tweens (20 and 80), Triton X-100, and EDTA increased the same. In addition, the study of the morphological alteration of chitin treated by enzyme by SEM revealed cracks and pores on the chitin surface, indicating a potential application of this enzyme in several industries.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacillus
/
Chitinases
Type of study:
Prognostic study
/
Qualitative research
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2016
Type:
Article
Affiliation country:
Iran
Institution/Affiliation country:
National Institute of Genetic Engineering and Biotechnology/IR
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