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In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
Zauli, Danielle Alves Gomes; Menezes, Carla Lisandre Paula de; Oliveira, Cristiane Lommez de; Mateo, Elvis Cristian Cueva; Ferreira, Alessandro Clayton de Souza.
  • Zauli, Danielle Alves Gomes; Instituto Hermes Pardini. Departamento de Pesquisa e Desenvolvimento. Vespasiano. BR
  • Menezes, Carla Lisandre Paula de; Instituto Hermes Pardini. Departamento de Pesquisa e Desenvolvimento. Vespasiano. BR
  • Oliveira, Cristiane Lommez de; Instituto Hermes Pardini. Departamento de Pesquisa e Desenvolvimento. Vespasiano. BR
  • Mateo, Elvis Cristian Cueva; Instituto Hermes Pardini. Departamento de Pesquisa e Desenvolvimento. Vespasiano. BR
  • Ferreira, Alessandro Clayton de Souza; Instituto Hermes Pardini. Departamento de Pesquisa e Desenvolvimento. Vespasiano. BR
Braz. j. microbiol ; 47(4): 987-992, Oct.-Dec. 2016. tab, graf
Article in English | LILACS | ID: biblio-828211
ABSTRACT
Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validation of the assay, an international panel of hepatitis B virus/hepatitis C virus and standard plasmids was used. A correlation coefficient of 0.983 and 0.963 for hepatitis B virus and hepatitis C virus, respectively, was obtained based on cycle threshold values and concentrations of DNA or RNA. The standard curve showed a linear relationship from 19 IU/mL to 1.9 × 109 IU/mL of serum, with a coefficient of determination (r2) of 0.99. In sera from patients infected with hepatitis B virus or hepatitis C virus viral loads (19 IU/mL and 1.9 × 109 IU/mL), we quantified viral loads with a detection limit of 1.9 × 102 IU/mL. The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients.
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Full text: Available Index: LILACS (Americas) Main subject: Hepatitis B virus / Hepatitis C / Hepacivirus / Viral Load / Hepatitis B Type of study: Diagnostic study Limits: Humans Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Hermes Pardini/BR

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Full text: Available Index: LILACS (Americas) Main subject: Hepatitis B virus / Hepatitis C / Hepacivirus / Viral Load / Hepatitis B Type of study: Diagnostic study Limits: Humans Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Hermes Pardini/BR