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In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes.
Porto, Karla Rejane de Andrade; Motti, Priscilla Rezende; Machado, Alexandre Alves; Roel, Antonia Railda.
  • Porto, Karla Rejane de Andrade; Universidade Federal de Mato Grosso do Sul. Programa de Pós-Graduação em Biotecnologia e Biodivesidade - Rede Pro Centro-Oeste. Campo Grande. BR
  • Motti, Priscilla Rezende; Universidade Federal de Mato Grosso do Sul. Programa de Pós-Graduação em Biotecnologia e Biodivesidade - Rede Pro Centro-Oeste. Campo Grande. BR
  • Machado, Alexandre Alves; Universidade Federal de Mato Grosso do Sul. Programa de Pós-Graduação em Biotecnologia e Biodivesidade - Rede Pro Centro-Oeste. Campo Grande. BR
  • Roel, Antonia Railda; Universidade Federal de Mato Grosso do Sul. Programa de Pós-Graduação em Biotecnologia e Biodivesidade - Rede Pro Centro-Oeste. Campo Grande. BR
Rev. Soc. Bras. Med. Trop ; 49(6): 693-697, Dec. 2016. tab, graf
Article in English | LILACS | ID: biblio-829668
ABSTRACT
Abstract

INTRODUCTION:

Dengue fever is a viral disease transmitted by the Aedes aegypti Linn. (1792) (Diptera Culicidae) mosquito, which is endemic in several regions of Brazil. Alternative methods for the control of the vector include botanical insecticides, which offer advantages such as lower environmental contamination levels and less likelihood of resistant populations. Thus, in this study, the ability of botanical insecticide formulations to inhibit the activity of the liver enzymes serum cholinesterase and malate dehydrogenase was evaluated.

METHODS:

Inhibition profiles were assessed using in vitro assays for cholinesterase and malate dehydrogenase activity and quantitated by ultraviolet-visible spectroscopy at 410nm to 340nm. RESULTS Insecticide products formulated from cashew nutshell liquid [A] and ricinoleic acid [B] showed cholinesterase activity levels of 6.26IU/mL and 6.61IU/mL, respectively, while the control level for cholinesterase was 5-12IU/mL. The products did not affect the level of 0.44IU/mL established for malate dehydrogenase, as the levels produced by [A] and [B] were 0.43IU/mL and 0.45IU/mL, respectively. CONCLUSIONS Our findings show that in vitro testing of the formulated products at concentrations lethal to A. aegypti did not affect the activity of cholinesterase and malate dehydrogenase, indicating the safety of these products.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Ricinoleic Acids / Cholinesterase Inhibitors / Cholinesterases / Anacardium / Insecticides / Liver / Malate Dehydrogenase Limits: Animals / Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Mato Grosso do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Ricinoleic Acids / Cholinesterase Inhibitors / Cholinesterases / Anacardium / Insecticides / Liver / Malate Dehydrogenase Limits: Animals / Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Mato Grosso do Sul/BR