Detección del gen mecA en cepas de Staphylococcus coagulasa positiva aisladas desde gatos / Detection of the mecA gene in coagulase positive Staphylococcus isolated from cats
Rev. chil. infectol
;
33(4): 410-418, ago. 2016. ilus, tab
Article
in Spanish
| LILACS
| ID: biblio-830111
ABSTRACT
Introduction:
Bacterial resistance is a global concern for public health. Reports of antimicrobial resistance, including that against methicillin, have increased in strains of coagulase positive Staphylococcus (CPS) isolated from pets, however in Chile this information is limited.Objectives:
To determine the antimicrobial susceptibility profiles and to detect the mecA gene in CPS strains isolated from cats in Chile. Materials andMethods:
134 samples were obtained from healthy cats and cats with skin lesions. These strains were characterized in their coagulase production and identified by BBL Crystal kit. The antimicrobial susceptibility was determined by Kirby Bauer method against 12 antimicrobials, including oxacillin. All strains were subjected to PCR to detect the mecA gene.Results:
72 CPS strains were isolated, including S. aureus and S. intermedius. Antimicrobial resistance against at least one drug was detected in strains from both healthy cats (75%) and from cats with skin lesions (87.5%). The mecA gene was detected in eight methicillin-resistant strains and also in three sensitive strains, being in general multi-resistant.Discussion:
These results highlight the role of pets as reservoirs of bacterial resistance, and their potential impact on national public health.RESUMEN
Introducción:
La resistencia bacteriana constituye un tema de preocupación para la salud pública mundial. Últimamente han aumentado los reportes de resistencia a antimicrobianos, incluida meticilina, en cepas de Staphylococcus coagulasa positiva (SCP) aisladas desde mascotas. Sin embargo, en Chile esta información es escasa.Objetivos:
Determinar el perfil de susceptibilidad antimicrobiana y detectar el gen mecA en cepas de SCP aisladas desde gatos en Chile. Materiales yMétodos:
Se obtuvieron 134 muestras desde gatos sanos y con lesiones dermatológicas. Las cepas fueron caracterizadas en su producción de coagulasa e identificadas mediante kit BBL Crystal. La susceptibilidad antimicrobiana se determinó mediante el método de Kirby Bauer ante 12 antimicrobianos, incluida oxacilina. Todas las cepas fueron sometidas a RPC para la detección del gen mecA.Resultados:
72 cepas de SCP fueron aisladas, incluyendo S. aureus y S. intermedius. Se detectó resistencia antimicrobiana a al menos un antimicrobiano en cepas de gatos sanos (75%) y de gatos con lesiones cutáneas (87,5%). El gen mecA fue detectado en ocho cepas resistentes a meticilina y en tres cepas sensibles, siendo en general multi-resistentes.Discusión:
Estos resultados destacan el rol de las mascotas como reservorios de resistencia bacteriana y su potencial impacto en la salud pública.
Full text:
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Index:
LILACS (Americas)
Main subject:
Bacterial Proteins
/
Cats
/
Polymerase Chain Reaction
/
Penicillin-Binding Proteins
/
Methicillin-Resistant Staphylococcus aureus
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Chile
Language:
Spanish
Journal:
Rev. chil. infectol
Journal subject:
Communicable Diseases
Year:
2016
Type:
Article
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de Chile/CL
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