Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study
Clin. biomed. res
;
34(3): 297-306, 2014. graf, ilus
Article
in English
| LILACS
| ID: biblio-834463
ABSTRACT
Introduction:
Mouse models of emphysema are important tools for testing different therapeutic strategies. The aim of this study was to develop a mouse model of emphysema induced by different doses of elastase in order to produce different degrees of severity.Methods:
Thirty female mice (C57BL/6) were used in this study. Different doses of porcine pancreatic elastase were administered intratracheally once a week for four weeks, as follows 0.1 U (n=8), 0.15 U (n=7), and 0.2 U (n=7). Control mice (n=8) received 50 microL of sterile saline solution intratracheally. Lung mechanics were analyzed by plethysmography. Mean linear intercept and volume fraction occupied by collagen and elastic fibers were determined.Results:
An increase in lung resistance was observed with 0.2 U of elastase [median (P-25-P75) 2.02 (1.67; 2.34) cmH2O.s/mL], as well as a decrease in tidal volume and minute ventilation. Peak expiratory flow increased significantly in the groups treated with 0.15 U and 0.2 U of elastase. Mean linear intercept was higher with 0.15 U and 0.2 U of elastase, with destruction of alveolar walls [median (P-25-P75) 30.31 (26.65-43.13) microm and 49.49 (31.67-57.71) microm respectively]. The volume fraction occupied by collagen and elastic fibers was lower in the group receiving 0.2 U of elastase.Conclusion:
Four intratracheal instillations of 0.2 U of elastase once a week induced changes in lung function and histology, producing an experimental model of severe pulmonary emphysema, whereas 0.15 U resulted in only histological changes.
Full text:
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Index:
LILACS (Americas)
Main subject:
Pulmonary Emphysema
/
Pancreatic Elastase
Limits:
Animals
Language:
English
Journal:
Clin. biomed. res
Journal subject:
Medicine
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Rio Grande do Sul/BR
/
Federal University of Rio Grande do Sul. Post Graduate Program in Surgical Sciences/BR
/
Federal University of Rio de Janeiro/BR
/
Hospital de Clínicas de Porto Alegre/BR
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