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Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant
Gomes, Rúdnei de Oliveira Luciano; Artigiani Neto, Ricardo; Guimarães Neto, José de Freitas; Nunes, Adriana Porto; Montero, Edna Frasson de Souza; Martins, José Luiz.
  • Gomes, Rúdnei de Oliveira Luciano; Universidade Federal de São Paulo. Postgraduate Program in Interdisciplinary Surgical Science. Sao Paulo. BR
  • Artigiani Neto, Ricardo; Universidade Federal de São Paulo. Postgraduate Program in Interdisciplinary Surgical Science. Sao Paulo. BR
  • Guimarães Neto, José de Freitas; Universidade Federal de São Paulo. Postgraduate Program in Interdisciplinary Surgical Science. Sao Paulo. BR
  • Nunes, Adriana Porto; Universidade Federal de São Paulo. Postgraduate Program in Interdisciplinary Surgical Science. Sao Paulo. BR
  • Montero, Edna Frasson de Souza; Universidade Federal de São Paulo. Postgraduate Program in Interdisciplinary Surgical Science. Sao Paulo. BR
  • Martins, José Luiz; Universidade Federal de São Paulo. Postgraduate Program in Interdisciplinary Surgical Science. Sao Paulo. BR
Acta cir. bras ; 32(3): 236-242, Mar. 2017. graf
Article in English | LILACS | ID: biblio-837688
ABSTRACT
Abstract

Purpose:

To evaluate the effect of remote ischemic preconditioning (r-IPC) administered to pregnant rats, in the ileum of newborn rats subjected to hypoxia and reoxygenation.

Methods:

We used three pregnant rats and their newborn rats distributed in three groups 1) Control (C) - Newborn rats born from a pregnant rat which did not undergo any intervention; 2) Hypoxia-Reoxygenation (H/R) - Newborn rats born from a pregnant rat which did not undergo any intervention, and were subjected to hypoxia-reoxygenation; 3) Remote Ischemic Preconditioning (r-IPC) - newborn rats born from a pregnant rat which was subjected to remote ischemic preconditioning twenty-four hours before giving birth and the newborn rats were subjected to hypoxia-reoxygenation. Segments of ileum were prepared for histological analysis by HE and immunohistochemistry by the Ki67 to evaluate cell proliferation, crypt depth and villus height and evaluation of apoptosis by cleaved caspase-3.

Results:

The intensity of the lesions was lower in the r-IPC than in the H/R group, showing significant difference (p<0.01). The r-IPC group showed a higher proliferative activity compared to the H/R group (p<0.01), with deeper crypts (r-IPC > H/R - p < 0.05), and higher villi, showing significant difference (r-IPC > H/R - (p <0.01). The occurrence of apoptosis in the H/R group was lower in comparison to groups C and r-IPC, with significant difference (H/R < r-IPC; p<0.05).

Conclusion:

The remote ischemic preconditioning applied to the pregnant rat protected the ileum of newborn rats subjected to hypoxia and reoxygenation, with decreased intensity of the lesions in the ileum mucosa and preservation of proliferative activity, keeping the villus height and crypt depth similar to group C.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Ischemic Preconditioning / Enterocolitis, Necrotizing / Ileum Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Ischemic Preconditioning / Enterocolitis, Necrotizing / Ileum Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de São Paulo/BR