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Naringenin regulates production of matrix metalloproteinases in the knee-joint and primary cultured articular chondrocytes and alleviates pain in rat osteoarthritis model
Wang, CC; Guo, L; Tian, FD; An, N; Luo, L; Hao, RH; Wang, B; Zhou, ZH.
  • Wang, CC; Dalian University. Department II of Orthopedics. Dalian. CN
  • Guo, L; Dalian University. Department II of Orthopedics. Dalian. CN
  • Tian, FD; Dalian University. Department II of Orthopedics. Dalian. CN
  • An, N; Dalian University. Department II of Orthopedics. Dalian. CN
  • Luo, L; Dalian University. Department II of Orthopedics. Dalian. CN
  • Hao, RH; Dalian University. Department II of Orthopedics. Dalian. CN
  • Wang, B; Dalian University. Department II of Orthopedics. Dalian. CN
  • Zhou, ZH; Dalian University. Department II of Orthopedics. Dalian. CN
Braz. j. med. biol. res ; 50(4): e5714, 2017. tab, graf
Article in English | LILACS | ID: biblio-839285
ABSTRACT
Inflammation of cartilage is a primary symptom for knee-joint osteoarthritis. Matrix metalloproteinases (MMPs) are known to play an important role in the articular cartilage destruction related to osteoarthritis. Naringenin is a plant-derived flavonoid known for its anti-inflammatory properties. We studied the effect of naringenin on the transcriptional expression, secretion and enzymatic activity of MMP-3 in vivo in the murine monosodium iodoacetate (MIA) osteoarthritis model. The assessment of pain behavior was also performed in the MIA rats. The destruction of knee-joint tissues was analyzed microscopically. Moreover, the effect of naringenin was also studied in vitro in IL-1β activated articular chondrocytes. The transcriptional expression of MMP-3, MMP-1, MMP-13, thrombospondin motifs (ADAMTS-4) and ADAMTS-5 was also studied in primary cultured chondrocytes of rats. Naringenin caused significant reduction in pain behavior and showed marked improvement in the tissue morphology of MIA rats. Moreover, a significant inhibition of MMP-3 expression in MIA rats was observed upon treatment with naringenin. In the in vitro tests, naringenin caused a significant reduction in the transcriptional expression, secretion and enzymatic activity of the studied degradative enzymes. The NF-κB pathway was also found to be inhibited upon treatment with naringenin in vitro. Overall, the study suggests that naringenin alleviated pain and regulated the production of matrix-metalloproteinases via regulation of NF-κB pathway. Thus, naringenin could be a potent therapeutic option for the treatment of osteoarthritis.
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Full text: Available Index: LILACS (Americas) Main subject: Arthralgia / Matrix Metalloproteinase 3 / Chondrocytes / Osteoarthritis, Knee / Flavanones / Knee Joint / Anti-Inflammatory Agents Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Dalian University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Arthralgia / Matrix Metalloproteinase 3 / Chondrocytes / Osteoarthritis, Knee / Flavanones / Knee Joint / Anti-Inflammatory Agents Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Dalian University/CN