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Analysis of microbial diversity in Shenqu with different fermentation times by PCR-DGGE
Liu, Tengfei; Jia, Tianzhu; Chen, Jiangning; Liu, Xiaoyu; Zhao, Minjie; Liu, Pengpeng.
  • Liu, Tengfei; Liaoning University of Traditional Chinese Medicine. College of Pharmacy. Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine. Dalian. CN
  • Jia, Tianzhu; Liaoning University of Traditional Chinese Medicine. College of Pharmacy. Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine. Dalian. CN
  • Chen, Jiangning; Liaoning University of Traditional Chinese Medicine. College of Pharmacy. Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine. Dalian. CN
  • Liu, Xiaoyu; Liaoning University of Traditional Chinese Medicine. College of Pharmacy. Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine. Dalian. CN
  • Zhao, Minjie; Liaoning University of Traditional Chinese Medicine. College of Pharmacy. Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine. Dalian. CN
  • Liu, Pengpeng; Liaoning University of Traditional Chinese Medicine. College of Pharmacy. Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine. Dalian. CN
Braz. j. microbiol ; 48(2): 246-250, April.-June 2017. tab, graf
Article in English | LILACS | ID: biblio-839375
ABSTRACT
Abstract Shenqu is a fermented product that is widely used in traditional Chinese medicine (TCM) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. The aim of this study was to investigate microbial diversity in Shenqu using different fermentation time periods. DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated that a strain of Pediococcus acidilactici (band 9) is the predominant bacteria during fermentation and that the predominant fungi were uncultured Rhizopus, Aspergillus oryzae, and Rhizopus oryzae. In addition, pathogenic bacteria, such as Enterobacter cloacae, Klebsiella oxytoca, Erwinia billingiae, and Pantoea vagan were detected in Shenqu. DGGE analysis showed that bacterial and fungal diversity declined over the course of fermentation. This determination of the predominant bacterial and fungal strains responsible for fermentation may contribute to further Shenqu research, such as optimization of the fermentation process.
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Full text: Available Index: LILACS (Americas) Main subject: Bacteria / Plant Extracts / Polymerase Chain Reaction / Denaturing Gradient Gel Electrophoresis / Biota / Fungi Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Liaoning University of Traditional Chinese Medicine/CN

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Full text: Available Index: LILACS (Americas) Main subject: Bacteria / Plant Extracts / Polymerase Chain Reaction / Denaturing Gradient Gel Electrophoresis / Biota / Fungi Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Liaoning University of Traditional Chinese Medicine/CN