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Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
Martins, Maira de S. N; Castro, Alessandra M. M. G. de; Lima, Michele dos S; Pinto, Vivian da S. C; Silva, Thaís G. da; Fava, Claudia Del; Depes, Claudio Regis; Okuda, Liria H; Pituco, Edviges M.
  • Martins, Maira de S. N; Instituto Biológico. Laboratório de Viroses de Bovídeos. São Paulo. BR
  • Castro, Alessandra M. M. G. de; Instituto Biológico. Laboratório de Viroses de Bovídeos. São Paulo. BR
  • Lima, Michele dos S; Instituto Biológico. Laboratório de Viroses de Bovídeos. São Paulo. BR
  • Pinto, Vivian da S. C; Instituto Biológico. Laboratório de Viroses de Bovídeos. São Paulo. BR
  • Silva, Thaís G. da; Instituto Biológico. Laboratório de Viroses de Bovídeos. São Paulo. BR
  • Fava, Claudia Del; Instituto Biológico. Laboratório de Viroses de Bovídeos. São Paulo. BR
  • Depes, Claudio Regis; Instituto Biológico. Laboratório de Viroses de Bovídeos. São Paulo. BR
  • Okuda, Liria H; Instituto Biológico. Laboratório de Viroses de Bovídeos. São Paulo. BR
  • Pituco, Edviges M; Instituto Biológico. Laboratório de Viroses de Bovídeos. São Paulo. BR
Braz. j. microbiol ; 48(2): 366-372, April.-June 2017. tab, graf
Article in English | LILACS | ID: biblio-839381
ABSTRACT
Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
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Full text: Available Index: LILACS (Americas) Main subject: Phylogeny / Molecular Diagnostic Techniques / Herpesviridae / Malignant Catarrh Type of study: Diagnostic study / Qualitative research Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Biológico/BR

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Full text: Available Index: LILACS (Americas) Main subject: Phylogeny / Molecular Diagnostic Techniques / Herpesviridae / Malignant Catarrh Type of study: Diagnostic study / Qualitative research Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Biológico/BR