Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
Braz. j. microbiol
;
48(2): 366-372, April.-June 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-839381
ABSTRACT
Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Phylogeny
/
Molecular Diagnostic Techniques
/
Herpesviridae
/
Malignant Catarrh
Type of study:
Diagnostic study
/
Qualitative research
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2017
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Biológico/BR
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