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Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus
Ma, Jianping; Wang, Jufang; Liu, Yanfen; Wang, Changyi; Duan, Donghui; Lu, Nanjia; Wang, Kaiyue; Zhang, Lu; Gu, Kaibo; Chen, Sihan; Zhang, Tao; You, Dingyun; Han, Liyuan.
  • Ma, Jianping; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Wang, Jufang; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Liu, Yanfen; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Wang, Changyi; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Duan, Donghui; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Lu, Nanjia; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Wang, Kaiyue; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Zhang, Lu; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Gu, Kaibo; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Chen, Sihan; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Zhang, Tao; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • You, Dingyun; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
  • Han, Liyuan; Shenzhen Nanshan Center for Chronic Disease Control. Department of Chronic Disease Prevention and Control. CN
Clinics ; 72(2): 111-115, Feb. 2017. tab, graf
Article in English | LILACS | ID: biblio-840040
ABSTRACT

OBJECTIVES:

The aim of this study was to compare the expression levels of serum miRNAs in diabetic retinopathy and type 2 diabetes mellitus.

METHODS:

Serum miRNA expression profiles from diabetic retinopathy cases (type 2 diabetes mellitus patients with diabetic retinopathy) and type 2 diabetes mellitus controls (type 2 diabetes mellitus patients without diabetic retinopathy) were examined by miRNA-specific microarray analysis. Quantitative real-time polymerase chain reaction was used to validate the significantly differentially expressed serum miRNAs from the microarray analysis of 45 diabetic retinopathy cases and 45 age-, sex-, body mass index- and duration-of-diabetes-matched type 2 diabetes mellitus controls. The relative changes in serum miRNA expression levels were analyzed using the 2-ΔΔCt method.

RESULTS:

A total of 5 diabetic retinopathy cases and 5 type 2 diabetes mellitus controls were included in the miRNA-specific microarray analysis. The serum levels of miR-3939 and miR-1910-3p differed significantly between the two groups in the screening stage; however, quantitative real-time polymerase chain reaction did not reveal significant differences in miRNA expression for 45 diabetic retinopathy cases and their matched type 2 diabetes mellitus controls.

CONCLUSION:

Our findings indicate that miR-3939 and miR-1910-3p may not play important roles in the development of diabetic retinopathy; however, studies with a larger sample size are needed to confirm our findings.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: MicroRNAs / Diabetes Mellitus, Type 2 / Diabetic Retinopathy Limits: Animals / Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Shenzhen Nanshan Center for Chronic Disease Control/CN

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Full text: Available Index: LILACS (Americas) Main subject: MicroRNAs / Diabetes Mellitus, Type 2 / Diabetic Retinopathy Limits: Animals / Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Shenzhen Nanshan Center for Chronic Disease Control/CN