Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus
Clinics
;
72(2): 111-115, Feb. 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-840040
ABSTRACT
OBJECTIVES:
The aim of this study was to compare the expression levels of serum miRNAs in diabetic retinopathy and type 2 diabetes mellitus.METHODS:
Serum miRNA expression profiles from diabetic retinopathy cases (type 2 diabetes mellitus patients with diabetic retinopathy) and type 2 diabetes mellitus controls (type 2 diabetes mellitus patients without diabetic retinopathy) were examined by miRNA-specific microarray analysis. Quantitative real-time polymerase chain reaction was used to validate the significantly differentially expressed serum miRNAs from the microarray analysis of 45 diabetic retinopathy cases and 45 age-, sex-, body mass index- and duration-of-diabetes-matched type 2 diabetes mellitus controls. The relative changes in serum miRNA expression levels were analyzed using the 2-ΔΔCt method.RESULTS:
A total of 5 diabetic retinopathy cases and 5 type 2 diabetes mellitus controls were included in the miRNA-specific microarray analysis. The serum levels of miR-3939 and miR-1910-3p differed significantly between the two groups in the screening stage; however, quantitative real-time polymerase chain reaction did not reveal significant differences in miRNA expression for 45 diabetic retinopathy cases and their matched type 2 diabetes mellitus controls.CONCLUSION:
Our findings indicate that miR-3939 and miR-1910-3p may not play important roles in the development of diabetic retinopathy; however, studies with a larger sample size are needed to confirm our findings.
Full text:
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Index:
LILACS (Americas)
Main subject:
MicroRNAs
/
Diabetes Mellitus, Type 2
/
Diabetic Retinopathy
Limits:
Animals
/
Humans
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2017
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Shenzhen Nanshan Center for Chronic Disease Control/CN
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